Abstract
Objective: To observe whether there is evidence for vascular channel formation by osteosarcoma cellsin vitro and to illustrate mechanism of vasculogenic mimicry in osteosarcoma.Methods: Osteosarcoma cell lines (U-2OS) were tested for their ability to form tubular networks in three-dimensional culture containing type I collagen. The structures of the tubular networks were observed under a phase contrast microscope and an electron microscope.Results: Observation under light microscopy and electron microscopy showed that high aggressive osteosarcoma cells line (U-2OS) formed networks containing channels when grown in three-dimensional culture containing type I collagen in the absence of endothelial cells or fibroblasts.Conclusion: These observations strongly suggest that aggressive osteosarcoma cells may generate vascular channels that facilitate tumor perfusion independent of tumor angiogenesis and have the ability of vasculogenic mimicry.
Similar content being viewed by others
References
Cai XS, Mei J, Li RL,et al. Clinical significance of microvessels density in osteosarcoma. J Clinical Orthopaedics, 1999, 2: 95–97.
Risau W. Mechanisms of angiogenesis. Nature, 1997, 386: 671–674.
Asahara T, Masuda H, Takahashi T,et al. Bone marrow origin of endothelial progenitor cells responsible for postnatal vasculogenesis in physiological and pathological neovascularization. Circ Res, 1999, 85: 221–228.
Maniotis AJ, Folberg R, Hess A,et al. Vascular channel formation by human melanoma cellsin vivo andin vitro: vasculogenic mimicry. Am J Pathol, 1999, 155: 739–752.
Ponten J, Saksela E. Two establishedin vitro cell lines from human mesenchymal tumors. Int J Cancer, 1967, 2: 434–447.
Montesano R, Orci L, Vassalli P.In vitro rapid organization of endothelial cells in to capillary-like networks is promoted by collagen matrices. J Cell Biol, 1983, 97: 1648–1652.
Khanna C, Khan J, Nguyen P. Metastasis-associated differences in gene expression in a murine model of osteosarcoma. Cancer Res, 2001, 61: 3750–3759.
Zhao Y, Qiu, GX. Relationship between vascular endothelial growth factor expression and angiogenesis in osteosarcoma. Acta. Academiae Medicinae Sinicae, 2000, 29: 430–432.
Bissell MJ. Tumor plasticity allows vasculogenic mimicry, a novel form of angiogenic switch. A rose by any other name? Am J Pathol, 1999, 155: 675–679.
Author information
Authors and Affiliations
Corresponding author
Additional information
This study was supported in part by the National Natural Sciences Foundation of China (No. 30271314).
Rights and permissions
About this article
Cite this article
Jiong, M., Yongwei, J. & Xuansong, C. Vascular channel formation by osteosarcoma cells in vitro: Vasculogenic mimicry. Chin. -Ger. J. Clin. Oncol. 2, 237–239 (2003). https://doi.org/10.1007/BF02835467
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF02835467