Abstract
Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas.
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References
Mosmann TR, Cherwinski H, Bond MW,et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol, 1986, 136: 2348–2357.
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extration. Anal Biochem, 1987, 162: 156–159.
Lederer JA, Liou JS, Todd MD,et al. Regulation of cytokine gene expression in T helper cell subsets. J Immunol, 1994, 152: 77–86.
Romagnani S. Type 1 T helper and type 2 T helper cells: functions, regulation and role in protection and disease. Intl J Clin Lab Res, 1991, 21: 152–158.
Kharkevitch DD, Seito D, Balch GC,et al. Characterization of autologous tumor-specific T-helper 2 cells in tumor-infiltrating lymphocytes from a patient with metastatic melanoma. Int J Cancer, 1994, 58: 317–323.
Huang M, Wang J, Lee P,et al. Human non-small cell lung cancer cells express a type 2 cytokines pattern. Cancer Res, 1995, 55: 3847–3853.
Kishimoto T. The biology of interleukin-6. Blood, 1989, 74: 1–10.
Van Meir E, Sawamura Y, Diserens AC,et al. Human glioblastoma cells release IL-6in vivo andin vitro. Cancer Res, 1990, 50: 6683–6688.
Li G, Li XG, Zhang QL,et al. The expression of IL-6 gene in human glioma cells and the certification of IL-6 autocrine circle. Chin J Neurosur, 2000, 16: 232–234.
Blay JY, Burdin N, Rousset F,et al. Serum interleukin-10 in non-Hodgkin’s lymphoma: a prognostic factor. Blood, 1993, 82: 2169–2174.
Roszman T, Elliott L, Brooks W. Modulation of T-cell function by gliomas. Immunol Today, 1991, 12: 370–374.
Hu YS, Zhang QL, Tian ZG,et al. Influence of adjustment of balance of Th1/Th2 type cytokines on proliferation of glioma cells. Acta Academiae Medicinae Sinicae, 2001, 23: 599–602.
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This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).
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Gang, L., Yongsheng, H., Xingang, L. et al. The role of predominant expression of Th2 type cytokines gene in the genesis and development of human gliomas. Chin. -Ger. J. Clin. Oncol. 2, 227–230 (2003). https://doi.org/10.1007/BF02835464
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DOI: https://doi.org/10.1007/BF02835464