Advertisement

The Chinese-German Journal of Clinical Oncology

, Volume 2, Issue 4, pp 227–230 | Cite as

The role of predominant expression of Th2 type cytokines gene in the genesis and development of human gliomas

  • Li Gang
  • Hu Yongsheng
  • Li Xingang
  • Zhang Qinglin
  • Jia Deze
  • Gong Songfeng
Original Articles

Abstract

Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas.

Key words

glioma Th1/Th2 gene expression RT-PCR 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Mosmann TR, Cherwinski H, Bond MW,et al. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol, 1986, 136: 2348–2357.PubMedGoogle Scholar
  2. 2.
    Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extration. Anal Biochem, 1987, 162: 156–159.CrossRefPubMedGoogle Scholar
  3. 3.
    Lederer JA, Liou JS, Todd MD,et al. Regulation of cytokine gene expression in T helper cell subsets. J Immunol, 1994, 152: 77–86.PubMedGoogle Scholar
  4. 4.
    Romagnani S. Type 1 T helper and type 2 T helper cells: functions, regulation and role in protection and disease. Intl J Clin Lab Res, 1991, 21: 152–158.CrossRefGoogle Scholar
  5. 5.
    Kharkevitch DD, Seito D, Balch GC,et al. Characterization of autologous tumor-specific T-helper 2 cells in tumor-infiltrating lymphocytes from a patient with metastatic melanoma. Int J Cancer, 1994, 58: 317–323.CrossRefPubMedGoogle Scholar
  6. 6.
    Huang M, Wang J, Lee P,et al. Human non-small cell lung cancer cells express a type 2 cytokines pattern. Cancer Res, 1995, 55: 3847–3853.PubMedGoogle Scholar
  7. 7.
    Kishimoto T. The biology of interleukin-6. Blood, 1989, 74: 1–10.PubMedGoogle Scholar
  8. 8.
    Van Meir E, Sawamura Y, Diserens AC,et al. Human glioblastoma cells release IL-6in vivo andin vitro. Cancer Res, 1990, 50: 6683–6688.PubMedGoogle Scholar
  9. 9.
    Li G, Li XG, Zhang QL,et al. The expression of IL-6 gene in human glioma cells and the certification of IL-6 autocrine circle. Chin J Neurosur, 2000, 16: 232–234.Google Scholar
  10. 10.
    Blay JY, Burdin N, Rousset F,et al. Serum interleukin-10 in non-Hodgkin’s lymphoma: a prognostic factor. Blood, 1993, 82: 2169–2174.PubMedGoogle Scholar
  11. 11.
    Roszman T, Elliott L, Brooks W. Modulation of T-cell function by gliomas. Immunol Today, 1991, 12: 370–374.CrossRefPubMedGoogle Scholar
  12. 12.
    Hu YS, Zhang QL, Tian ZG,et al. Influence of adjustment of balance of Th1/Th2 type cytokines on proliferation of glioma cells. Acta Academiae Medicinae Sinicae, 2001, 23: 599–602.PubMedGoogle Scholar

Copyright information

© Springer 2003

Authors and Affiliations

  • Li Gang
    • 1
  • Hu Yongsheng
    • 2
  • Li Xingang
    • 1
  • Zhang Qinglin
    • 1
  • Jia Deze
    • 1
  • Gong Songfeng
    • 1
  1. 1.Department of NeurosurgeryQilu Hospital of Shandong UniversityJinanChina
  2. 2.Department of NeurosurgeryBeijing Xuan Wu HospitalBeijingChina

Personalised recommendations