Summary
Comparative mapping, or ascertaining the gene linkage relationships between different species, is rapidly developing. This is possible because new techniques in chromosome identification and somatic cell hybridization, such as the generation of hybrids preferentially segregating chromosomes of any desired species including rodents, and the development of gene transfer techniques have yielded new information about the human and rodent gene maps. In addition, the discovery and characterization of mouse subspecies has generated new mouse sexual genetic linkage data. The following picture is emerging. Several X-linked genes in man are X-linked in all mammalian species tested. The linkage relationships of several tightly linked genes, less than 1 map unit apart, are also conserved in all mammalian species tested. Ape autosomal genes are assigned to ape chromosomes homologous to their human counterparts indicating extensive conservation in the 12 million years (MYR) of evolution from apes to man. Similarly, mouse and rat, 10 MYR apart in evolution, have several large autosomal synteny groups conserved. In comparing the mouse and human gene maps we find that human genes assigned to different arms of the same human chromosome are unlinked in the mouse; mouse genes large map distances (20 to 45 cM) apart are very likely to be unlinked in the human. However, several autosomal synteny groups 10 to 20 cM apart, including thePgd, Eno-1, Pgm-1 group on human chromosome arm lp, are conserved in mice and man. This suggests that homology mapping, the superimposition of one species gene map on the homologous conserved portion of another species genome may be possible, and that ancestral autosomal synteny groups should be detectable.
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References
Bergsma, D. (Ed.). 1976.Birth Defects (Original Article Series). Baltimore Conferences, Third International Workshop on Human Gene Mapping, 1975, Vol. XII, No. 7. The National Foundation, New York.
Warburton, D., and P. L. Pearson. 1976. Report of the committee on comparative mapping. In: D. Bergsma (Ed.),Birth Defects (Original Article Series). Baltimore Conferences. Third International Workshop on Human Gene Mapping, 1975. Vol. XII, No. 7. The National Foundation, New York, pp. 75–82.
Wilson, A. C., L. R. Maxson, and V. M. Sareich. 1974. Two types of molecular evolution. Evidence from studies of interspecific hybridization. Proc. Natl. Acad. Sci. U.S.A. 71: 2843–2847.
Wilson, A. C., V. M. Sareich, and L. R. Maxson. 1974. The importance of gene rearrangement in evolution: evidence from studies on rates of chromosomal, protein, and anatomical evolution. Proc. Natl. Acad. Sci. U.S.A. 71: 3028–3030.
Wilson, A. C., G. L. Bush, S. M. Case, and M. C. King. 1975. Social structuring of mammalian populations and rate of chromosomal evolution. Proc. Natl. Acad. Sci. U.S.A. 72: 5061–5065.
Comings, D. E. 1972. Evidence for ancient tetraploidy and conservation of linkage groups in mammalian chromosomes. Nature 238: 455–457.
Ohno, S. 1973. Ancient linkage groups and frozen accidents. Nature 244: 259–262.
Rice, N. R. 1972. In: H. H. Smith (Ed.),Evolution of Genetic Systems. Gordon and Breach, New York, pp. 44–79.
Benveniste, R. E., and G. J. Todaro. 1976. Evolution of type C viral genes: evidence for an Asian origin of man. Nature 261: 101–108.
Miller, O. R., and D. A. Miller. 1975. Cytogenetics of the mouse. Ann. Rev. Genet. 9: 285–303.
Rotterdam Conference (1974). 1975.Second International Workshop on Human Gene Mapping. Birth Defects (Original Article Series, The National Foundation, New York). Vol. XI. No. 3.
Minna, J. D., and H. G. Coon. 1974. Human x mouse hybrid cells segregating mouse chromosomes and isozymes. Nature 252: 401–404.
Minna, J. D., T. H. Marshall, and P. V. Shaffer-Berman. 1975. Chinese hamster x mouse hybrid cells segregating mouse chromosomes and isozymes. Som. Cell Genet. 1: 355–369.
Minna, J. D., T. H. Marshall, and P. Shaffer-Berman. 1976. Gene mapping by somatic cell hybridization in species other than man. In: D. Bergsma (Ed.),Birth Defects (Original Article Series). Baltimore Conferences. Third International Workshop on Human Gene Mapping, 1975. Vol. Xii, No. 7. The National Foundation, New York, pp. 422–429.
Minna, J. D., J. Yavelow, and H. G. Coon. 1975. Expression of phenotypes in hybrid somatic cells derived from the nervous system. Genetics Supplement. Proceedings XIIIth Congress of Genetics 79: 373–383.
Kozak, C., E. Nichols, and F. H. Ruddle. 1975. Gene linkage analysis in the mouse by somatic cell hybridization: assignment of adenine phosphoribosyltransferase to chromosome 8 andα-galactosidase to the X chromosome. Som. Cell Genet. 1: 371–382.
Deisseroth, A., and A. Nienhuis. 1976. Study of markers of erythroid differentiation in hybrid cells. In Vitro 12: 734–742.
Deisseroth, A. 1976. Isolation of hybrid cells that exhibit markers of erythroid differentiation. N. Engl. J. Med 294: 148–152.
McBride, O. W. Permanent transfer of genes to eukaryotic cells by uptake of metaphase chromosomes. In Vitro, in press.
Womack, J. E., and M. Sharp. 1976. Comparative autosomal linkage in mammals: genetics of esterases inMus musculus andRattus norvegicus. Genetics 82: 665–675.
Chapman, V. M. 1975. 6 phosphogluconate dehydrogenase (PGD) genetics in the mouse: linkage with metabolically related enzyme loci. Biochem. Genet. 13: 849–856.
Searle, A. G. 1976. Mouse mutant gene list. Mouse News Lett. 54: 6–22.
Womack, J. E. 1976. Linkage map of the mouse. Mouse News Lett. 55: 6
Womack, T. E., N. L. Hawes, E. R. Soures, and T. H. Roderick. 1976. Mitochondrial malate dehydrogenase (Mor-1) in the mouse: linkage to chromosome 5 markers. Biochem. Genet., in press.
Chapman, V. M., and T. B. Shows. 1976. Somatic cell genetic evidence for X-chromosome linkage of three enzymes in the mouse. Nature 259: 665–667.
Turleau, C., J. de Grouchy, and M. Klein. 1972. Phylogenic chromosomique de l’homme et des primates hominiens (Pan troglodytes, Gorilla gorilla etPongo pygmaeus). Essai de reconstitution du caryotype de l’ancetre commun. Ann. Genet. 15: 225–40.
Dutrillaux, B., M. O. Rethore, M. Prieur, J. Lejeune. 1973. Analyse de la structure fine des chromosomes du Gorille (Gorilla gorilla): comparison avecHomo sapiens etPan troglodytes. Humangenetik 20: 343–354.
Warburton, D., I. L. Firschein, D. A. Miller, and F. E. Warburton. 1973. Karyotype of the chimpanzee,Pan troglodytes, based on measurements and banding patterns: comparison to the human karyotype. Cytogenet. Cell Genet. 12: 453–461.
Hood, L., J. H. Campbell, and S. C. R. Elgin. 1975. The organization, expression, and evolution of antibody genes and other multigene families. Ann. Rev. Genet. 9: 305–353.
Willecke, K., R. Lange, A. Kruger, and T. Reber. 1976. Cotransfer of two linked human genes into cultured mouse cells. Proc. Natl. Acad. Sci. U.S.A. 73: 1274–1278.
Nesbitt, M. 1974. Evolutionary relationships between rat and mouse chromosomes. Chromosoma 46: 217–24.
Womack, J., Davisson, E. Eicher, and D. Kendall. 1976. Mouse News Lett. 54: 41.
Bruns, G. A. P., and P. S. Gerald. 1976. Expression of the human adenylate kinase isozymes, phosphopyruvate hydratase, 6-phosphogluconate dehydrogenase, and phosphoglucomutase-1 in man-rodent somatic cell hybrids. Biochem. Genet. 14: 1–17.
Lalley, P. A., U. Francke, and J. D. Minna. Unpublished data.
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Minna, J.D., Lalley, P.A. & Francke, U. Comparative mapping using somatic cell hybrids. In Vitro 12, 726–733 (1976). https://doi.org/10.1007/BF02835447
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DOI: https://doi.org/10.1007/BF02835447