Apoptosis induced by Bcl-2 antisense peptide acid in HL60 cells
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To study the differences and similarties of the antisense drugs with different structures on the biological functions of HL60 cells.
Cytotoxic effects were measured by cell viability assay. The expression levels of protein were assayed by immunofluorecence using fluoresce isothiocyanate label. The morphological changes in apoptotic cells were observed. Flow cytometric analysis of DNA fragmentation was also performed.
Antisense peptide nucleic acid (PNA) targeting the coding region of the Bcl-2 mRNA could effectively inhibit the growth of HL60 cells, down-regulate the synthesis of Bcl-2 protein and induce apoptosis. After HL60 cells were treated with 10 μmol/L Bcl-2 antisense PNA or antisense oligonucleotide for 72 h respectively, apoptotic rates of HL60 cells were 17.80±1.53 and 13.17±1.12, respectively (P<0.05).
Antisense PNA targeting the coding region of Bcl-2 mRNA may have stronger antisense effects than the antisense oligonucleotides and could induce apoptosis of HL60 cells.
Key wordsBcl-2 antisense peptide nucleic acid antisense oligonucleotide HL60 cells apoptosis
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