Spacer length variation in rice 5S rRNA genes revealed by polymerase chain reaction

  • Yi Qingming
  • Liu Guoping


Polymerase chain reaction (PCR) was used to amplify 5S rRNA spacer from wild rice (Oryza rufipogon and O. nivara) and cultivated rice (indica and japonica varieties of O. sativa L). The results show that there is spacer length variation within and between species, and the typical indica and japonica varieties have their unique banding patterns of amplified 5S rRNA spacers, whereas intermediate showed no specific amplification profile of spacer regions. The 5S rRNA genes in intermediate are either identical with that of indica variety or that of japonica variety. These data suggest that the spacer length polymorphisms can be used to distinguish between closely ralated species and subspecies.

Key words

Rice 5S rRNA genes PCR 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Scoles G J, Gill B S, Xin Z Y,et al. Frequent duplication and deletion events in the 5SRNA genes and the associated spacer region in the Triticea.Pl Syst Evol, 1988,160: 105–122CrossRefGoogle Scholar
  2. 2.
    Doyle J J, Brown A H D. 5S ribosomal gene variation in the Glycine tomentalla polyploid complex (leguminosae).Syst Bot, 1989,14: 398–407CrossRefGoogle Scholar
  3. 3.
    Dvorak J, Zhang H B, Kota R S,et al. Origination and evolution of the 5S ribosomal RNA gene family in wheat and related species.Genome, 1989,32: 1003–1016Google Scholar
  4. 4.
    Reddy P, Apples R. A second locus for the 5S multigene family in secale L: sequence divergence in two lineages of the family.Genome, 1989,32: 456–467Google Scholar
  5. 5.
    Cox A V, Bennet M D, Dyer T A. Use of the polymerase chain reaction to detect spacer size heterogenecity in plant 5S rRNA gene clusters and to locate such clusters in wheat (Triticum aestivum L).Theor Appl Genet, 1992,83: 684–690CrossRefGoogle Scholar
  6. 6.
    Moran G F, Smith D, Bell J C,et al. The 5S rRNA genes in pinus ratiata and the the spacer region as a probe for relationships between pinus species.Pl Syst Evol, 1992,183: 209–221CrossRefGoogle Scholar
  7. 7.
    Hu J, Quiros C F. Identification of broccoli and cauliflower cultivars with RAPD markers.Plant Cell Report, 1991,10: 505–511Google Scholar
  8. 8.
    Bassam B J, Caetano-Anoiles G, Gresshoff P. M. Fast and sensitive silver staining of DNA in polyacrylamide gel.Anal Biochem, 1991,196: 80–83CrossRefGoogle Scholar
  9. 9.
    Second G. Evolutionary relationships in the sativa group of Oryza based on isozymdata.Genet Sel Evol, 1985;17: 89–94CrossRefGoogle Scholar
  10. 10.
    Yi Q M, Deng W G, Xia Z P,et al. Polymorphism and genetic relatedness among wild and cultivated rice species determined by AP-PCR analysis.Hereditas, 1995,122: 135–141CrossRefGoogle Scholar

Copyright information

© Springer 1997

Authors and Affiliations

  • Yi Qingming
    • 1
  • Liu Guoping
    • 1
  1. 1.College of Life SciencesWuhan UniversityWuhanChina

Personalised recommendations