Advertisement

Role of nuclear factor kappaB in intestine injury induced by hepatic ischemia reperfusion

  • Chen Junhua
  • Wang Guobin
Article

Summary

The role of nuclear factor kappaB in intestine injury induced by hepatic ischemia reperfusion was investigated. Eighteen male Wistar rats were divided into 3 groups randomly: sham operation group (group A), hepatic ischemia reperfusion group (group B) and hepatic ischemia reperfusion plus pyrrolidine dithiocarbamate (PDTC) group (group C). The rats in group A were only subjected to laparotomy, those in group B underwent partial hepatic ischemia reperfusion (ischemia for 1 h and reperfusion for 2 h) and those in group C underwent the same procedure as that of group B but received PDTC 200 mg/kg i. v. before and after ischemia. After reperfusion, tissues of jejunum and venous blood were obtained for measurement of TNF-α, MDA and MPO. The levels of TNF-α in jejunum and venous blood, the levels of MPO in jejunum in group B were significantly higher than those in group A and group C (P<0.05). There was no significant different in the levels of MDA between group B and group C. The severity of histological intestinal injury in group B and group C was similar. Hepatic ischemia reperfusion caused intestine injury, NF-kappaB may play an important role in this course and the targeting of upstream components of the inflammatory response, such as NF-kappaB, may have important therapeutic applications.

Key words

liver ischemia reperfusion nuclear factor kappaB rats 

References

  1. 1.
    Liu D L, Jeppsson B, Hakansson C Het al. Multiplesystem organ damage resulting from prolonged hepatic flow interruption. Arch Surg, 1996, 131: 442PubMedGoogle Scholar
  2. 2.
    Ghosh S, May M J, Kopp E Bet al. Rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol, 1998, 16: 225PubMedCrossRefGoogle Scholar
  3. 3.
    Kobayashi H, Nonami T, Kurokawa Tet al. Mechanism and prevention of ischemia reperfusion induced liver injury in rats. J Surg Res, 1991, 51: 240PubMedCrossRefGoogle Scholar
  4. 4.
    Lentsch A B, Yoshidome H, Cheadle W Get al. Chemokine involvement in hepatic ischemia reperfusion injury in mice: roles for macrophage inflammatory protein 2 and KC. Hepatology, 1998, 27: 1172PubMedCrossRefGoogle Scholar
  5. 5.
    Peralta C, Fernandez L, Panes Jet al. Preconditioning protects against systemic disorders associated with hepatic ischemia reperfusion through blockade of tumor necrosis factor-α induced P-selectin up-regulation in the rat. Hepatology. 2001, 33: 100PubMedCrossRefGoogle Scholar
  6. 6.
    Collins T, Read M A, Neish A Set al. Transcriptional regulation of endothelial cell adhesion molecules: NF-κB and cytokine-inducible enhancers. FASEBJ, 1995, 9: 899Google Scholar
  7. 7.
    Zwacka R M, Zhang Y, Zhou Wet al. Ischemia reperfusion injury in the liver of BALB/c mice activates AP-1 and nuclear factorκB independently of IkB degradation. Hepatology, 1998, 28: 1022PubMedCrossRefGoogle Scholar
  8. 8.
    Van Antwarp D J, Martin S J, Verma I Met al. Inhibition of TNF induced apoptosis by NF-κB. Trends Cell Biol, 1998, 8: 107CrossRefGoogle Scholar

Copyright information

© Springer 2004

Authors and Affiliations

  • Chen Junhua
    • 1
  • Wang Guobin
    • 1
  1. 1.Department of General Surgery, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

Personalised recommendations