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In Vitro

, Volume 16, Issue 2, pp 159–167 | Cite as

Comparison of collagen and glycosaminoglycan synthesis in attaching control and diabetic human skin fibroblasts

  • R. Edward Branson
  • Kenneth J. Lembach
  • Leon W. Cunningham
Article

Summary

Cultured fibroblasts derived from normal subjects and juvenile diabetics attach in the absence of serum to plastic culture dishes and secrete macromolecules, including collagenous components, hyaluronic acid, and proteoglycans into the medium and onto the plastic surface where they form a microexudate carpet. Most diabetic fibroblasts examined did not spread as well as normal cells during a 4-hr interval after the initial attachment. There were no significant differences between normal and diabetic cells with respect to proline and lysine incorporation and lysine hydroxylation. The percentage glycosylation of hydroxylysine was marginally higher in the media proteins of diabetic cells, but glycosylation in both normal and diabetic cells was elevated over that typically observed in human skin collagen.

Collagenous components were estimated to constitute approximately 15–20% of the microexudate carpet fraction in both normal and diabetic cell strains. Diabetic fibroblasts exhibited a marginally lower ratio of heparan sulfate to chondroitin sulfate in the cell surface to matrix microexudate carpet fraction (trypsinate) than did normal fibroblasts. The hyaluronate and chondroitin sulfate contents of this fraction of diabetic cells were not significantly different from those of normal cells.

Keywords

diabetes fibroblasts collagen glycosaminoglycans 

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Copyright information

© Tissue Culture Association 1980

Authors and Affiliations

  • R. Edward Branson
    • 1
  • Kenneth J. Lembach
    • 1
  • Leon W. Cunningham
    • 1
  1. 1.Department of BiochemistryVanderbilt University School of MedicineNashville

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