Effects of 2-APB on store-operated Ca2+ channel currents of hepatocytes after hepatic ischemia/reperfusion injury in rats

  • Huang Changzhou
  • Zhang Zongming
  • Qiu Fazu


The effects of hepatic ischemia/reperfusion (I/R) injuries on hepatocellular viability and store-operated calcium current (Isoc) in isolated rat hepatocytes and the effects of 2-APB on store-operated calcium current (Isoc) in isolated rat hepatocytes after hepatic ischemia/reperfusion injuries were studied. Hepatic ischemia and reperfusion injury model was establised and whole cell patch-clamp techniques were used to investigate the effects of 2-APB on Isoc. The results showed that ischemia/reperfusion injuries could significantly reduce hepatocellular viability and further increase Isoc in hepatocyte and 2-APB (20, 40, 60, 80, 100 μmol/L) produced a concentration-dependent decrease of Isoc with IC50 value of 64.63±10.56 μmol/L (n=8). It was concluded that ischemia/ reperfusion injuries could reduce hepatocellular viability, probably through increased Isoc in hepatocytes and 2-APB had a protective effect on ischemia/reperfusion-induced liver injury, probably though inhibiting Isoc.

Key words

hepatic-ischemia reperfusion injuries hepatocytes store-operated calcium current store-operated calcium channel calcium channel blockers 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Huang Changzhou
    • 1
  • Zhang Zongming
    • 2
  • Qiu Fazu
    • 1
  1. 1.Department of Surgery, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Surgery, The First Af filiated HospitalQinghua UniversityBeijingChina

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