Skip to main content
SpringerLink
Log in

Effects of 2-APB on store-operated Ca2+ channel currents of hepatocytes after hepatic ischemia/reperfusion injury in rats

  • Published:
Current Medical Science Aims and scope Submit manuscript

Summary

The effects of hepatic ischemia/reperfusion (I/R) injuries on hepatocellular viability and store-operated calcium current (Isoc) in isolated rat hepatocytes and the effects of 2-APB on store-operated calcium current (Isoc) in isolated rat hepatocytes after hepatic ischemia/reperfusion injuries were studied. Hepatic ischemia and reperfusion injury model was establised and whole cell patch-clamp techniques were used to investigate the effects of 2-APB on Isoc. The results showed that ischemia/reperfusion injuries could significantly reduce hepatocellular viability and further increase Isoc in hepatocyte and 2-APB (20, 40, 60, 80, 100 μmol/L) produced a concentration-dependent decrease of Isoc with IC50 value of 64.63±10.56 μmol/L (n=8). It was concluded that ischemia/ reperfusion injuries could reduce hepatocellular viability, probably through increased Isoc in hepatocytes and 2-APB had a protective effect on ischemia/reperfusion-induced liver injury, probably though inhibiting Isoc.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Putney J W Jr, McKay R R. Capcitative calcium entry channels. Bioessays, 1999, 21:38

    Article  PubMed  Google Scholar 

  2. Barritt G J. Receptor-activated Ca2+ inflow in animal cells: a variety of pathways tailored to meet different intracellular Ca2+ signaling requirements. Biochem J, 1999, 337:153

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Fierro L, Parekh A B. Substantial depletion of the intracellular Ca2+ store is require for macroscopic activation of the Ca2+ release-activated Ca2+ current in rat basophilic leukemia cell. J Physiol, 2000, 522(2):247

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Rychkov G, Brereton H M, Harland M Let al. Plasma membrane Ca2+ release-activated Ca2+ channel with a high selectivity for Ca2+ identified by patch-clamp recording in rat liver cells. Hepatology, 2001, 33:938

    Article  CAS  PubMed  Google Scholar 

  5. Maruyama T, Kanaji T, Nakade Set al. 2-APB, 2-aminoethoxydiphenyl borate, a membrane-penetrable modulator of ins (1,4,5) P3-induce Ca2+ release. J Biochem, 1997, 122(3):498

    Article  CAS  PubMed  Google Scholar 

  6. Iwasaki H, Mori Y, Uchida Ket al. 2-aminoethoxydiphenyl borate (2-APB) inhibits capacitative calcium entry independently of the function of inositol 1, 4, 5-trisphospate receptors. Receptors Channels, 2001, 7(6): 429

    CAS  PubMed  Google Scholar 

  7. Bootman M D, Collins T J, Mackenzie Let al. 2-aminoethoxydiphenyl borate (2-APB) is a reliable blocker of store-operated calcium entry but an inconsistent inhibitor of ins (1,4,5) P3-induce Ca2+ release. FASEB J, 2002, 16(10):1145

    Article  CAS  PubMed  Google Scholar 

  8. Colletti L M, Remick D G, Burtch G Det al. Role of tumor necrosis factor-alpha in the pathophysiologic alterations after hepatic ischemia/reperfusion injury in the rat. J Clin Invest, 1990, 85(6):1936

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Alpini G, Phillips J O, Vroman Bet al. Recent advances in the isolation of liver cells. Hepatology, 1994, 20(2):494

    Article  CAS  PubMed  Google Scholar 

  10. Barros L F, Stutzin A, Calixto Aet al. Nonselective cation channel as effectors of free radical-induced rat liver cell necrosis. Hepatology, 2001, 33(1):114

    Article  CAS  PubMed  Google Scholar 

  11. Fan C, Zwacka R M, Engelhardt J F. Therapeutic approaches for ischemia/reperfusion injury in the liver. J Mol Med, 1999, 77:577

    Article  CAS  PubMed  Google Scholar 

  12. Losser M R, Payen D. Mechanisms of liver damage. Semin Liver Dis, 1996, 16(4): 357

    Article  CAS  PubMed  Google Scholar 

  13. Broad L M, Braun F J, Livremont J Pet al. Role of phospholipase C-inositol 1, 4, 5-trisphosphate pathway in calcium release-activated calcium current and capacitative calcium entry. J Biol Chem, 2001, 276(19): 15 945

    Article  CAS  Google Scholar 

  14. Trebak M, St J Bird G, McKay R Ret al. Signaling mechanism for receptor-activated canonical transient receptor potential 3 (TRPC3) channels. J Biol Chem, 2003, 278(18):16 244

    Article  CAS  Google Scholar 

  15. Chapham D E, Runnels L W, Strubing C. The TRP ion channel family. Nature Reviews Neuroscience, 2001, 2 (6): 387

    Article  Google Scholar 

  16. Ma H T, Patterson R L, Van Rossum D Bet al. Requirement of the inositol triphosphate receptor for activation of store-operated Ca2+ channels. Science, 2000, 287(5458): 1647

    Article  ADS  CAS  PubMed  Google Scholar 

  17. Kukkonen J P, Lund P E, Akerman K E. 2-aminoethoxydiphenylborate reveals heterogeneity in receptor-activated Ca2+ discharge and store-operated Ca2+ influx. Cell Calcium, 2001, 30(2): 117

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 43003, Wuhan, China

    Huang Changzhou & Qiu Fazu

  2. Department of Surgery, The First Af filiated Hospital, Qinghua University, 100016, Beijing, China

    Zhang Zongming

Authors
  1. Huang Changzhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhang Zongming
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Qiu Fazu
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Huang Changzhou, male, born in 1965, M. D., Ph.D.

This project was supported by the National Natural Science Foundation of China (No. 30270532), the Trans-Century Excellent Talent Development Plan Fund of Ministry of Education of China (Official Letter No. 2002-48) and Shuguang Program Project of Shanghai Educational Committee (No. 02SG20).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Changzhou, H., Zongming, Z. & Fazu, Q. Effects of 2-APB on store-operated Ca2+ channel currents of hepatocytes after hepatic ischemia/reperfusion injury in rats. Current Medical Science 25, 39–41 (2005). https://doi.org/10.1007/BF02831382

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02831382

Key words

Search

Navigation