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Expression of Cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor

  • Huang Yinping
  • Ye Duyun
  • Wu Ping
  • Huang Yanjun
  • Zhang Li
  • Zhou Xiaoyan
  • Huang Yunfeng
  • Yuan Ping
  • Zhang Daijuan
  • Wan Jingyuan
Article

Summary

In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.

Key words

cyclooxygenase-2 gene splice variant gene cloning sequencing myometrium 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Huang Yinping
    • 1
  • Ye Duyun
    • 2
  • Wu Ping
    • 2
  • Huang Yanjun
    • 1
  • Zhang Li
    • 2
  • Zhou Xiaoyan
    • 2
  • Huang Yunfeng
    • 2
  • Yuan Ping
    • 2
  • Zhang Daijuan
    • 2
  • Wan Jingyuan
    • 2
  1. 1.Department of Obstetrics and GynecologyFirst Affiliated Hospital of Wenzhou Medical CollegeWenzhouChina
  2. 2.Department of Pathophysiology, School of Basic Medical Sciences, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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