Summary
In order to investigate the expression of cyclooxygenase-2 (COX-2) in human lower segments of myometrium obtained from women in labor and those not in labor and identify the splicing variant of COX-2, reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of COX-2. The primers were designed and synthesized according to the sequence of rat COX-2 splice variant which was discovered firstly by us. Then the splicing variant of COX-2 in human myometrium from woman in labor was identified, cloned into vector and sequenced. The results showed that the expression of COX-2 mRNA was lower in human myometrium obtained from women who were not in labor than that in labor women and a new band of COX-2 was obtained in myometrium from labor woman. The fragment included an unspliced intron, which pitched between exons 7 and 8. It was suggested that COX-2 gene was not only expressed highly in human myometrium from woman in labor, but also produced splicing variant by alternative splicing.
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HUANG Yinping, female, born in 1954, professor
This project was supported by grants from National Natural Sciences Foundation of China (No. 30070929, 30271363).
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Yinping, H., Duyun, Y., Ping, W. et al. Expression of Cyclooxygenase-2 mRNA and identification of its splice variant in human myometrium obtained from women in labor. Current Medical Science 25, 5–7 (2005). https://doi.org/10.1007/BF02831372
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DOI: https://doi.org/10.1007/BF02831372