Analysis of male sterility-related proteins of young panicle in rice (Oryza sativa L.) by two-dimensional electrophoresis

  • Hu Yao-jun
  • Wei Lei
  • Liu Shu-nan
  • Yu Jin-hong
  • Ding Yi


For searching out male sterility-related proteins (polypeptides) in rice (Oryza sativa L.), we examined the difference of panicle protein (polypeptides) between hybrid rice (Wujin2A/R168, Wujin5A/R988) and their parents (male-sterile line Wujin2A, Wujin5A, and restorer line R168, R988) at the formation stage of pollen mother cell by two-dimensional electrophoresis (2-DE). The results revealed that the 2-DE polypeptide maps were similar among these experimental materials. A small group of polypeptides were disappeared in 2-DE polypeptide maps of male-sterile line (Wujin2A, Wujin5A) by comparing to restorer line (R168, R988) and the first filial (F1) generation (Wujin2A/R168, Wujin5A/R988). The isoelectric points of these polypeptides were pI 5.8–6.5 molecular, weight 42.7×103−66.2×103.

Key words

hybrid rice male-sterile protein two-dimensional polyacrylamide gel electrophoresis 

CLC number

Q 946.1 


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  1. [1]
    Zhu Ying-guo, Li Rong-qian, Wang Ming-quan.Biology of Male Sterility in Rice. Wuhan: Wuhan University Press, 2000. 1–29 (Ch).Google Scholar
  2. [2]
    Yuan Long-ping. Current Status and Developing Prospects in Two-Line Hybrid Rice Research in China,Research of Agricultural Modernizanation, 1997,18(1):1–3 (Ch).Google Scholar
  3. [3]
    Yuan Long-ping, Wang San-liang.Science of Hybrid Rice. Bejing: China Agriculture Press, 2002, 38–78 (Ch).Google Scholar
  4. [4]
    Zivy M, Vienne D D. Protemics: A Link Between Genetics and Physiology.Plant Mol Bio, 2000,44:575–580.CrossRefGoogle Scholar
  5. [5]
    Moons A, Prinsen E, Bauw G,et al. Molecular and Physiological Responses to Abscisic Acid and Salts in Roots of Salt-Sensitive and Salt-TolerantIndica Rice Varieties.Plant Physiol, 1995,107:177–186.CrossRefGoogle Scholar
  6. [6]
    Moons A, Bauw G, Prinsen E,et al. Antagonistic Effects of Abscisic Acid and Jasmonates on Salt Stress-Inducible Transcripts in Rice Roots.Plant Cell, 1997,9:2243–2259.CrossRefGoogle Scholar
  7. [7]
    Dameral C, Le Guilloux M. Characterization of Novel Proteins Affected by the O-2 Mutation and Expressed During Maize Endosperm Development.Mol Gen Genet, 1998,257:354–361.CrossRefGoogle Scholar
  8. [8]
    Zhong Bo-xiong. A New Method for Analysis of Rice Protein Using Two-Dimensional Electrophoresis.,J Zhejiang Agricultural Univ, 1997,23(2):128–132 (Ch).Google Scholar
  9. [9]
    Komatsu S, Rakwal R, Zhijun L. Separation and Characterization of Proteins in Rice (Oryza sativa L.) Suspension Cultured Cells.Plant Cell, Tissue and Organ Culture, 1999,55:183–192.CrossRefGoogle Scholar
  10. [10]
    He Rui-feng, Ding Yi, Yu Jin-hong. Leaf Protein Analysis in the Temperature-Sensitive Chlorophyll Deficient Rice Mutants by Two-Dimensional Electrophoresis.Acta Agronomica Sinica, 2001,27(6):875–879 (Ch).Google Scholar
  11. [11]
    Sun Tae-kim, Kyu Seong-cho, Yu Sin-jang.,et al. Two-Dimensional Electrophoresis Analysis of Rice Proteins by Polyethylene Glycol Fraction for Protein Arrays.,Electrophoresis, 2001,22(10):2103–2109.CrossRefGoogle Scholar
  12. [12]
    Imin N, Kerim T, Weinman J J,et al. Characterization of Rice Anther Proteins Expressed at the Young Microspore Stage.Proteomics, 2001,9:1149–1161.CrossRefGoogle Scholar
  13. [13]
    Wei Lei, Ding Yi, Hu Yao-jun,et al. Analysis of Leaf Proteins of Zidao Male-Sterile Line by 2D-PAGE.Acta Genetica Sinica, 2002,29(8):696–699 (Ch).Google Scholar
  14. [14]
    Yuan Long-ping.A Concise Course in Hybrid Rice. Changsha: Hunan Sci and Tech Press, 1985. 83–92.Google Scholar
  15. [15]
    Feng Jiu-huan, Lu Yong-gen, Liu Xiang-dong,et al. Pollen Development and Its Stages in Rice (Oryza sativa L.).Chinese J Rice Sci, 2001,15(1):21–28 (Ch).Google Scholar
  16. [16]
    He Rui-feng, Ding Yi, Zhang Jian-feng,et al. Improvement in the Two-Dimensional Electrophoresis of Proteins from the Leaves of Plant.,Hereditas, 2000,22(5):319–321 (Ch).Google Scholar
  17. [17]
    Celis J E, Ratz G, Basse B,et al. High-Resolution Two-Dimensional Gel Electrophoresis of Proteins: IEF and NEPH-GE. in:Cell Biology—A Laboratory Handbook. San Diego: Academic Press, 1998. 375–385.Google Scholar
  18. [18]
    Liu Jia-yao, Luo Jai-ling, Liang Zheng. Some Improvement of TD Page Technology.Chinese Bulletin of Botanica, 1998,15(3):69–72 (Ch).Google Scholar
  19. [19]
    Yang Wei, Bi Xue-zhi, Huang Da-nian,et al. A Method for the Two-Dimensional Gel Electrophoresis, of Rice Proteins.Chinese J Rice Sci, 1993,7(1):43–47 (Ch).Google Scholar
  20. [20]
    Cao Yi-cheng, Fu Bin-ying, Wang Ming-quan,et al. A Comparative Study on Proteins of the HPGMR by Two-Dimensional Electrophoresis.J Wuhan Univ (Nat Sci Ed), 1987, (HPGMR Suppl): 73–80 (Ch).Google Scholar
  21. [21]
    Huang Qing-liu, Tang Xi-hua, Mao Jian-lei. Effects of Temperature on Pollen Fertility and Anther Protein in Thermo-Sensitive Genic Male-Sterile Rice.Acta Phytophysiologica Sinica, 1996,22(1):69–73 (Ch).Google Scholar
  22. [22]
    Zhu Ying-guo, Yu Jing-hong, Xu Shu-hua,et al. Studies on Ma Xie A of Ma Wei Zhan CMS in Native Rice Variety of China.J Wuhan Univ (Nat Sci Ed), 1993, (6):110–115 (Ch).Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Hu Yao-jun
    • 1
    • 2
  • Wei Lei
    • 1
  • Liu Shu-nan
    • 1
    • 3
  • Yu Jin-hong
    • 1
  • Ding Yi
    • 1
  1. 1.Key Laboratory of MOE for Plant Developmental Biology/College of Life SciencesWuhan UniversityWuhan, HubeiChina
  2. 2.College of Life SciencesHunan University of Science and TechnologyXiangtan, HunanChina
  3. 3.College of Life SciencesHuazhong Normal UniversityWuhan, HubeiChina

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