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Relationship between the expression of connexin43 and bystander effect of suicide gene therapy in ovarian cancer

  • Zhang Ali
  • Wang Quansheng
  • Han Zhiqiang
  • Wu Sufang
  • Chen Gang
  • Li Jun
  • Liao Guoning
  • Lu Yunping
  • Ma Ding
Article

Summary

The relationship of connexin43 (Cx43) and bystander effect in ovarian tumor cells in herpes simplex virus thymidine kinase/ganciclovir (HSV-TK/GCV) gene therapyin vitro was explored and the effect of all-trans retinoic acid (RA) on the expression of Cx43 and bystander effect investigated. The Cx43 expression was detected by flowcytometry, Western, blot, and immunofluorescence in two ovarian tumor cell lines OVCAR3, CaOV3 before and after RA treatment. Bystander effect was determined by the cells growth inhibitory rate with methyl, thiazolyl tetrazolium. Following exposure to ganciclovir, there was much greater bystander killing in OVCAR3 than that in CaOV3 (P<0.05). The expression of Cx43 was detected in OVCAR3 by flowcytometry and Western blot, but it could not be detected in CaOV3. The expression of Cx43 in both cell lines could be induced by RA. Immunofluorescence staining showed that Cx43 protein of OVCAR3 was located on membrane surface, whereas CaOV3 in cytoplasm. RA could not change the location of Cx43 protein in both cell lines. There is relationship between Cx43 expression and HSV-TK/GCV bystander effect. HSV-TK/GCV bystander effect can be enhanced by RA in ovarian cancer.

Key words

gene therapy ovarian neoplasms connexin43 retinoic acid 

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Copyright information

© Springer 2004

Authors and Affiliations

  • Zhang Ali
    • 1
  • Wang Quansheng
    • 2
  • Han Zhiqiang
    • 1
  • Wu Sufang
    • 1
  • Chen Gang
    • 1
  • Li Jun
    • 1
  • Liao Guoning
    • 1
  • Lu Yunping
    • 1
  • Ma Ding
    • 1
  1. 1.Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Nephrology, Union Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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