HPLC determination of ascorbic acid using luminol-Cu (II) chemiluminescence-application to the analysis of Juice beverage
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The chemiluminescence (CL) of luminol-Cu (II) was applied to HPLC determination of ascorbic acid, which was separated by a C18 reverse-phase column with a mobile phase of 0.25 mol/L HAc. The eluted ascorbic acid was mixed with 0.3 mmol/L luminol and 0.05 mol/L CuSO4. The light emission from the reaction of Cu(II) oxidized ascorbic acid and luminol was detected by a modified luminometer. The detection limit was 3.6×10−6 mol/L for ascorbic acid at aS/N ratio of 3, and the linear calibration range was 2×10−4–2×10−3 mol/L. The relative standard deviation for 5 replicate injections of 1×10−3 mol/L ascorbic acid was calculated as 4.3%. The method was successfully applied to determination of ascorbic acid in juice beverage.
Key wordsascorbic acid chemiluminescence luminol HPLC beverage
CLC numberO 657.3
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