Advertisement

Wuhan University Journal of Natural Sciences

, Volume 2, Issue 3, pp 363–367 | Cite as

Separation of DNA fragments by cross-linked polymer coating capillary electrophoresis with non-gel sieving matrix

  • Chen Hong
  • Song Liguo
  • Cheng Jieke
Article

Abstract

A new type of flush/fill equipment driven by high-pressure (1MPa) nitrogen gas for filling the capillary is demonstrated. High-viscous and microlitre grade volume solution can be filled into a capillary by means of this equipment. The capillary coated by cross-linked polyacrylamide and filled with “interpenetrating polymer networks” non-gels sieving matrix (0.8% hydroxyethyl cellulose, 10% sucrose in 1×TBE buffer) was applied for separating DNA fragments. All 11 fragments of Ø174/Hae III DNA were successfully separated. The man-made circle plasmid from E. coli were separated and detected.

Key words

capillary electrophoresis cross-linked polyacrylamide coating non-gels sieving matrix DNA fragments circle plasmid DNA 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cheng Jieke, Human genome and DNA sequence analysis-Past, present and future.J Anal Sci. (Chinese), 1994,10(3): 61–72Google Scholar
  2. 2.
    Xiong Shaoxiang, Li Jianjun, Cheng Jieke. Studies on the coupling technique of high performance capillary electrophoresis with charge-coupled device detector.J Anal Sci (Chinese), 1995,11(3): 12–15Google Scholar
  3. 3.
    Cheng J, Mitchelson K R. Glycerol-Entangled Separation of DNA Fragments in Entangled Solution Capillary Electrophoresis.Anal Chem, 1994,66: 4210–4214CrossRefGoogle Scholar
  4. 4.
    Hjenten S. Elimination of electroosmosis and solute adsorption.J Chromatogr A, 1985,347: 191–198CrossRefGoogle Scholar

Copyright information

© Springer 1997

Authors and Affiliations

  • Chen Hong
    • 1
  • Song Liguo
    • 1
  • Cheng Jieke
    • 1
  1. 1.Department of ChemistryWuhan UniversityWuhanChina

Personalised recommendations