Effects of CTLA4-Ig gene-modified dendritic cells on the corneal allografts
- 33 Downloads
In order to investigate the effects of mouse CTLA4Ig gene-modified dendritic cells (DCs) on the survival of the corneal allografts in rats, the plasmid PG/CTLA4Ig was transfected into DCs of F344 rats mediated by LipfectamineTM 2000. The expression of CTLA4Ig was detected by immunofluorescent microscopy. The effects of donor DCs on the proliferation of T cells in Lewis rats (recipients) were tested by by CCK8. Corneal transplantation was performed from F344 rats to Lewis rats. The DCs modified with CTLA4Ig gene were injected into the Lewis rats on the day 0 and 3 after transplantation. The movement of the DCs after modification in vivo was observed by immunofluorescent microscopy, and the survival of corneal allografts was evaluated by Holland criterion. The results showed that the CTLA4Ig-modified DCs could restrain the proliferation of allogenetic T cells. The CTLA4Ig-modified DCs prolonged survival of corneal allografts. (P<0.01). It was suggested that the injection of CTLA4Ig gene-modified DCs could obviously inhabit the allograft rejection and prolong the survival of corneal allografts.
Key wordsCTLA4Ig dendritic cell corneal transplantation rat
Unable to display preview. Download preview PDF.
- 2.Yang L H, Hao J H, Zhang X G. Langerhans cell of cornea. Guo Wai Yi Xue Yan Ke Xue Fen Ce (Chinese), 2004, 28: 12–15Google Scholar
- 3.Gong F L. Immunology (Chinese). Beijing: Sciences Press, 2000. 96–97Google Scholar
- 4.Williams K A, Coster D J. Penetrating corneal transplantation in the inbred rat: a new model. Invest Opthalmol Vis Sci, 1985, 26: 23–30Google Scholar
- 7.Wang Q M, Hu Y H, Xu H Met al. A study of alteration in CCL19 content and dendritic cell number in local-draining lymph nodes after penetrating keratoplasty. Chin J Optometry Ophthalmol (Chinese), 2005, 7: 80–83Google Scholar