Wuhan University Journal of Natural Sciences

, Volume 7, Issue 4, pp 493–498 | Cite as

Gene product expression of cyclin D2 and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy

  • Zhang Yu-xia
  • Yu Lun-yin
  • Liu Ming-qiu
  • Tang Zhi-jiao
  • Xia Dong
  • Wang Ming


The current study was to investigate mRNA expression of cyclin D2 and p16 during the transition from cardiac myocyte hyperplasia to hypertrophy. Cultured cardiac myocytes (CM) and fibroblasts (FC) obtained from 1-day-old Sparague-Dawley rats were used in this study. We have determined (1) hyperplasia by cell growth curve and fluorescence activated cell sorting (FACS); and (2) ultrastructure by electron microscope observation; and (3) expresions of cyclin D2 mRNA and p16 mRNA by using in situ hybridization and image analysis. The results were shown (1) Results of cell growth curve and FACS analysis showed CM could proliferate in the first 3 cultured days (4 days in postnatal development). But the ability decreased quickly, concomitant with the differentiation. (2) The ultrastructure of CM showed the large amount of myofilaments and mitochondrion and FC showed moderate amount of rough endoplasmic reticulum. (3) The expression of cyclin D2 mRNA in 3−, 4−, 5−day CM group was 0.89 times (p<0.05), 0.80 times (p<0.05) and 0.56 times (p<0.01) of that in 1-day group respectively. P16 mRNA in 2−, 3−, 4−, 5−day CM group were 1.63 times (p<0.01), 1.72 times (p<0.01), 1.99 times (p<0.01) and 2.84 times (p<0.01) of that in 1−day group respectively. It can be concluded that cultured neonatal rat cardiac myocytes could proliferate during the first 3 cultured days, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D2 and p16 have the key roles during the transition from myocyte hyperplasia to hypertrophy.

Key Words

cyclins gene p16 hyperplasia cardiac myocytes rat 

CLC number

R 361.3 


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Copyright information

© Springer 2002

Authors and Affiliations

  • Zhang Yu-xia
    • 1
  • Yu Lun-yin
    • 2
  • Liu Ming-qiu
    • 1
  • Tang Zhi-jiao
    • 1
  • Xia Dong
    • 1
  • Wang Ming
    • 1
  1. 1.Department of Pathology, School of MedicineWuhan UniversityWuhan, HubeiChina
  2. 2.Beth Israel Deaconess Medical CenterHarvard Medical SchoolBostonUSA

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