Skip to main content
Log in

Tumor necrosis factor-related apoptosis ligand induces apoptosis in prostate cancer PC-3M cell line

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

To study the effect of tumor necrosis factor-related apoptosis inducing ligand (TRAIL) on PC-3M cell line, PC-3M cell line was incubated with gradient concentrations of TRAIL for 4–24 h. Annixin-V fluorescence staining and TUNEL method were employed to detect the apoptosis of PC-3M cells. The morphology of apoptotic PC-3M cells was observed by electron microscopy. The relationship between TRAIL concentrations and the percentage of apoptotic cells was evaluated by flow cytometry. The proliferation inhibitory ratio was calculated by using MTT colorimetry. Our results showed that apoptosis of PC-3M cells could be induced by treatment with TRAIL for at most 4 h. The results of flow cytometry and MTT colorimetry demonstrated a time- and concentration-dependent relationship between cell apoptosis rate and TRAIL concentration. It is concluded that apoptosis of PC-3M cells can be induced by TRAIL. Because of the selective killing effect of TRAIL on tumor cells, it may become a potential alternative for the treatment of advanced prostate cancer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pan G, O'Rourke K, Chinnaiyan A Met al. The receptor for the cytotoxic ligand TRAIL. Science, 1997, 276 (5309):111

    Article  PubMed  CAS  Google Scholar 

  2. Pitti R M, Marsters S A, Ruppert Set al. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J Biol Chem, 1996, 271: 12687

    Article  PubMed  CAS  Google Scholar 

  3. Shetty S, Gladden J B, Henson E Set al. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) upregulates death receptor 5 (DR5) mediated by NFkap-paB activation in epithelial derived cell lines. Apoptosis, 2002, 7(3):413

    Article  PubMed  CAS  Google Scholar 

  4. Plasilova M, Zivny J, Jelinek Jet al. TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia, 2002, 16(1):67

    Article  PubMed  CAS  Google Scholar 

  5. Keane M M, Ettenberg S A, Nau M Met al. Chemotherapy augments TRAIL-induced apoptosis in breast cell lines. Cancer Res, 1999, 59(3):734

    PubMed  CAS  Google Scholar 

  6. Vignati S, Codegoni A, Polato Fet al. Trail activity in human ovarian cancer cells: potentiation of the action of cytotoxic drugs. Eur J Cancer, 2002, 38(1):177

    Article  PubMed  CAS  Google Scholar 

  7. Ozoren N, Kim K, Burns T Fet al. The caspase 9 inhibitor Z-LEHD-FMK protects human liver cells while permitting death of cancer cells exposed to tumor necrosis factor-related apoptosis-inducing ligand. Cancer Res, 2000, 60(22):6259

    PubMed  CAS  Google Scholar 

  8. Modur V, Nagarajan R, Evers B Met al. FOXO proteins regulate tumor necrosis factor-related apoptosis inducing ligand expression, implications for RTEN mutation in prostate cancer. J Biol Chem. 2002, 277(49):47928

    Article  PubMed  CAS  Google Scholar 

  9. Jenster G. Ligand-independent activation of the androgen receptor in prostate cancer by growth factors and cytokines. J Pathol, 2000, 191:227

    Article  PubMed  CAS  Google Scholar 

  10. Sheridan J P, Marsters S A, Pitti R Met al. Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors. Science, 1997, 277:818

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

CHEN Zhaohui, male, born in 1974, M. D., Ph.D.

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30070756).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhaohui, C., Huafang, W., Longjie, G. et al. Tumor necrosis factor-related apoptosis ligand induces apoptosis in prostate cancer PC-3M cell line. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 25, 442–444 (2005). https://doi.org/10.1007/BF02828218

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02828218

Key words

Navigation