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In vitro anti-tumor immune response induced by dendritic cells transfected with recombinant adenovirus carrying mutant k-ras genes

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Summary

The specific anti-tumor immune response induced by mouse bone marrow dendritic cells (DCs) transfected with recombinant adenovirus carrying mutant k-ras genes was investigated. DCs were generated from mouse bone marrow in the presence of rmGM-CSF (3.3 ng/mL) and rmIL-4 (1.3 ng/mL) and detected by FACS, and then transfected with the recombinant adenovirus encoding mutant k-ras gene. The efficacy of transfection and T cell stimulating activity of DCs were detected. CTL activity of the mice vaccinated with DCs was observed. The results showed that DCs had dendritic veiled morphology. BmDCs highly expressed B7-1 (80%), B7-2 (77%), MHC II (70%), CD11c (65%), CD40 (70%) and CD54 (96%) with FACS, and no significant difference in the expression was observed before and after the transfection (P>0.05). The DCs transfected by mutant k-ras gene could significantly stimulate lymphocytes proliferation as compared with those transfected by Ad-c or non-modified DCs (P<0.05). DC vaccine transfected by mutant k-ras gene could induce CTL activity against Lewis lung cancer, but not against B16. The specific cytotoxicity against Lewis lung cancer in Ad-k-ras/12-transduced DC group was significantly higher than those in the control, vector and non-transfected DCs groups (P<0.05). It was concluded that special antitumor response could be induced by DCs transfected with recombinant adenovirus carrying mutant k-ras genes.

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Authors and Affiliations

  1. Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China

    Zhao Feng, Li Jinsong & Wang Jianjun

  2. Cancer Center of West China Hospital, West China Medical School, Sichuan University, 610041, Chengdu, China

    Zhou Qinghua, Lu Yanrong, Qin Yang & Zhang Jie

Authors
  1. Zhao Feng
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  2. Zhou Qinghua
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  3. Lu Yanrong
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  4. Qin Yang
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  5. Zhang Jie
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  6. Li Jinsong
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  7. Wang Jianjun
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Additional information

Zhao Feng, male, born in 1971, Doctor in Charge, M. D., Ph.D.

This project was supported by a grant from National Sciences Foundation of China (No. 30000206).

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Feng, Z., Qinghua, Z., Yanrong, L. et al. In vitro anti-tumor immune response induced by dendritic cells transfected with recombinant adenovirus carrying mutant k-ras genes. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 25, 378–381 (2005). https://doi.org/10.1007/BF02828201

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  • DOI: https://doi.org/10.1007/BF02828201

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