Reversion mutation in dark variants of luminous bacteria and its application in gene toxicant monitoring

  • Guo Jianli
  • Sun Yaliang


The luminous intensity of dark variant (S1) separated from photobacterium phosphoreum (A2) was 1/10 000 less than that of wild-type. Ethidium bromide (EB) (0.6 mg/L), Mytomycin C (MC, 0.05 mg/L), 2-amino fluorene (2-AF, 1.0 mg/L) all could strongly induce reversion mutation for S1 within 24 h and increase reversion ratio significantly. The results of experiments indicated that these revertants had stable genetic characteristic and the mutation may take place at gene levels. The mutagenesis to S1 caused by EB, MC and 2-AF was detected and it may be used as a new rapid, simple and sensitive method for gene toxicant monitoring.

Key words

ethidium bromide mytomycin C 2-amino fluorine dark variant reversion mutation gene toxicant monitoring 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Nealson K H, Hasting J W. Bacterial bioluminescence. Microbiological Reviews, 1979, 43(4): 496PubMedGoogle Scholar
  2. 2.
    Ulitzur S. Bioluminescence test for mutagenic compounds. Mutation Research, 1980, 74: 113PubMedGoogle Scholar
  3. 3.
    Cal J, Miyamoto S C. Regulatory mutants of vibrio harveyi lux system. J Biolumin Chemilumin. 1989, 3: 207CrossRefGoogle Scholar
  4. 4.
    Clear C. Mutation assay in bacteria. Methods Mol Biol, 1995, 43: 297Google Scholar
  5. 5.
    Meighen E A. Molecular biology of bacterial bioluminescence. Microbiological Reviews, 1991, 55(1): 123PubMedGoogle Scholar
  6. 6.
    Weiser I, Ulitzur S. DNA-damaging agents induce luminescence in dark variants of luminous bacteria. Mutation Research, 1981, 91: 443PubMedCrossRefGoogle Scholar

Copyright information

© Springer 2002

Authors and Affiliations

  • Guo Jianli
    • 1
  • Sun Yaliang
    • 1
  1. 1.Department of Biochemistry, School of Basic Medical Sciences, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan

Personalised recommendations