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Biological characteristics of human bone marrow mesenchymal stem cell culturedin vitro

  • Fa Xian’en
  • Wang Lixia
  • Hou Jianfeng
  • Zhang Ruicheng
  • Wang Haiyong
  • Yang Chenyuan
Article

Summary

Some biological characteristics of human bone marrow mesenchymal stem cells (MSCs) culturedin vitro were observed. hMSCs were isolated from bone marrow and purified by density gradient centrifugation method, and then culturedin vitro. The proliferation and growth characteristics of hMSCs were observed in primary and passage culture. MSCs of passage 3 were examined for the purify by positive rate of CD29 and CD44 through flow cytometry. Human bone marrow MSCs showed active proliferation capacityin vitro. The purify of MSCs separated by our method was higher than 90%. It was concluded that hMSCs have been successfully cultured and expanded effectively. It provided a foundation for further investigation and application of MSCs.

Key words

mesenchymal stem cell bone marrow cell culture 

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Copyright information

© Springer 2005

Authors and Affiliations

  • Fa Xian’en
    • 1
    • 2
  • Wang Lixia
    • 2
  • Hou Jianfeng
    • 2
  • Zhang Ruicheng
    • 2
  • Wang Haiyong
    • 2
  • Yang Chenyuan
    • 1
  1. 1.Department of Cardiac Surgery, Union Hospital, Tongji Medical CollageHuazhong University of Science and TechnologyWuhanChina
  2. 2.Department of Cardiac SurgeryThe Secondary Hospital of Zhengzhou UniversityZhengzhouChina

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