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Down-regulation of cardiac bradykinin B2 receptors and eNOs mRNA in rats with remnant kidneys

  • Wan Huaibin
  • Tu Ling
  • Liu Xiaoqing
  • Deng Juanjuan
Article
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Summary

The changes in the expression of cardiac bradykinin B2 receptors (BKB2Rs) and endogenous nitrix oxide synthase (eNOs) mRNA were studied in rats with remnant kidneys. Thirty-two rats were divided into sham-operated and experimental groups randomly (n=16 in each group). The remnant kidney model was established by 2-stage 5/6 nephrectomy. Blood pressure and serum Cr were measured before operation and 15, 30, 60, 120 days after 5/6 nephrectomy. Eight animals in each group were killed at the first month and 4th month after the operation. The expression of BKB2Rs and eNOs mRNAs was detected by using RT-real time PCR from isolated left ventricle, and their correlation was also analyzed. The results showed that blood pressure and serum Cr were increased significantly 15 days after 5/6 nephrectomy (bothP<0.01), and the hypertension and azomia existed constantly till 120 days but had no significant fluctuation. Cardiac BKB2Rs and eNOs mRNA was declined time-dependently (bothP<0.05). And there was a close positive correlation between cardiac BKB2Rs and eNOs mRNA (r=0.82,P<0.01). It was suggested that a significant chronic renal failure can be produced at least 15 days after 5/6 nephrotomy and can sustain more than 4 months. The expression of BKB2Rs and eNOs was down-regulated time-dependently in this model, and there was a significant correlation between them.

Key words

bradykinin B2 receptors endogenous nitric oxide synthase myocardium 5/6 nephretomy 

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References

  1. 1.
    Marceau F, Hess J F, Bachvarov D R. The B1 receptors for kinins. Pharmacol Rev, 1998,50:357PubMedGoogle Scholar
  2. 2.
    Bhoola K D, Figueroa C D, Worthy K. Bioregulation of kinins: kallikreins, kininogens, and kininases. Pharmacol Rev, 1992,44:1PubMedGoogle Scholar
  3. 3.
    Nolly H, Carbini L A, Scicli Get al. A local kallikrein-kinin system is present in rat hearts. Hypertension, 1994,23:919PubMedGoogle Scholar
  4. 4.
    Hinojosa-Laborde C, Frolich B, Cowley A W Jr. Whole body autoregulation in reduced renal mass hypertension. Hypertension, 1992,20:659PubMedGoogle Scholar
  5. 5.
    Yayama K, Matsuoka S, Nagaoka Met al. Down-regulation of bradykinin B2-receptor mRNA in the heart in pressure-overload cardiac hypertrophy in the rat. Biochem Pharmacol, 2003,65(5):1017PubMedCrossRefGoogle Scholar
  6. 6.
    Bogin E, Massry S G, Harary I. Effect of parathyroid hormone on rat heart cells. J Clin Invest, 1981, 67(4): 1215PubMedCrossRefGoogle Scholar
  7. 7.
    Tan Y, Hutchison F N, Jaffa A A. Mechanisms of angiotensin II-induced expression of B2 kinin receptors. J Physiol Heart Circ Physiol, 2004, 286(3):H926CrossRefGoogle Scholar
  8. 8.
    Ritter O, Schuh K, Brede Met al. AT2 receptor activation regulates myocardial eNOS expression via the calcineurin-NF-AT pathway, FASEB J, 2003, 17(2): 283PubMedGoogle Scholar
  9. 9.
    Downey J M. The cellular mechanisms of ischeamic and pharmacological preconditioning. Cardiovasc J S Afr, 2004,15(4 Suppl 1):S3Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Wan Huaibin
    • 1
  • Tu Ling
    • 1
  • Liu Xiaoqing
    • 1
  • Deng Juanjuan
    • 1
  1. 1.Geriatric Department, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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