Effects of murine cytomegalovirus infection on sperm viability in mice

  • Xiong Jinwen
  • Xiong Chengliang
  • Tian Yonghong
  • Hu Lian
  • Wei Hai


In order to explore the effects of testicular infection of murine cytomegalovirus (MCMV) on mature sperm viability at different periods following MCMV inoculation in mice, 91 BALB/c mice without MCMV infection were randomly divided into two groups: an experimental group (n=56) and a control group (n=35). The mice in the experimental group were treated by inoculating MCMV intratesticularly, while those in the controlled group were directly inoculated with DMEM without MCMV. The mice in both groups were sacrificed separately on the day 1,1.5,2,4,6,9 and 14 post-inoculation (D1, 1.5,2,4,6,9 and 14 PI). The MCMV M83 mRNA gene was detected in the testis byin situ hybridization (ISH) with MCMV late-mRNA probe labeled with digoxin. Sperm viability of mature sperm in the epididymis cauda was measured. The results demonstrated the positive signal of ISH of MCMV was found mainly in the cytoplasm of the testicular interstitial cells and spermatogenic cells in the experimental group. Compared with that in the controlled group, the sperm viability in the experimental group was decreased significantly on D1 PI and D1.5 PI (P<0.05). No statistically significant difference in the sperm viability was found after D2 PI between two groups (P>0.05). This suggested that sperm viability in mice might be descended significantly shortly after MCMV infection and might return to normal with time, indicating that MCMV acute infection might temporarily degrade sperm quality and influence procreation transiently.

Key words

murine cytomegalovirus testis in situ hybridization sperm viability 


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  1. 1.
    Clifford D B. Opportunistic viral infections in the setting of human immunodeficiency virus. Semin Neurol, 1999, 19(2): 185–192PubMedGoogle Scholar
  2. 2.
    McGowan M P, Hayes K, Kovacs G Tet al. Prevalence of cytomegalovirus and Herps simplex virus in human semen. Int J Androl, 1983, 6: 331–336PubMedCrossRefGoogle Scholar
  3. 3.
    Chevalier M S, Daniels G M, Johnson D Cet al. Binding of human cytomegalovirus US2 to major histocompatibility complex class I and II proteins is not sufficient for their degradation. J Virol, 2002, 76(16): 8265–8275PubMedCrossRefGoogle Scholar
  4. 4.
    Xiong J W, Chen S H, Wen L Zet al. Study of the nest-polymerase chain reaction used in the CMV congenital infection in guinea pig model. Clin J Exp Clin Virol (Chinese), 2004, 18: 34Google Scholar
  5. 5.
    Pallier C, Tebourbi L, Stephanie C Pet al. Herpesvirus, cytomegalovirus, human sperm and assited fertilization. Human Reproduction, 2002, 17: 1281–1287PubMedCrossRefGoogle Scholar
  6. 6.
    Yang Y S, Ho H N, Chen H Fet al. Cytomegalovirus infection and viral shedding in the genital tract of infertile couples. J Med Virol, 1995, 45: 179–182PubMedCrossRefGoogle Scholar
  7. 7.
    Xiong J W, Xiong C L, Tian Y Het al. Effects of testis murine cytomegalovirus infection on sperm acrosome reaction and spermatic function of membrane in mice. National Med J China (Chinese), 2005, 85: 1714–1717Google Scholar
  8. 8.
    Tebourbi L, Courtot A M, Duchateau Ret al. Experimental inoculation of male mice with murine cytomegalovirus and effect on offspring. Human Reproduction, 2001, 16: 2041–2049PubMedCrossRefGoogle Scholar
  9. 9.
    Choi Y H, Landim-Alvarenga F L, Seidel G Eet al. Effect of capacitation of stallion sperm with polyvinylalcohol or bovine serum albumin on penetration of bovine zona-free or partially zona-removed equine cocytes. J Ani Sci, 2003, 81: 2082–2087Google Scholar
  10. 10.
    Lang D J, Kummer J F. Demonstration of cytomegalovirus in semen. N Engl J Med, 1972, 287: 756–758PubMedCrossRefGoogle Scholar
  11. 11.
    Young J A, Cheung K S, Lang D J. Infection and fertilization of mice after artificial insemination with a mixture of sperm and murine cytomegalovirus. J Infect Dis 1977, 135: 837–840PubMedGoogle Scholar
  12. 12.
    Krause W, Herbstreit F, Slenzka Wet al. Are viral infections the cause of leukocytospermia?. Andrologia, 2002, 34: 87–90PubMedCrossRefGoogle Scholar
  13. 13.
    Baskar J F, Stanat S C, Huang E S. Murine cytomegalovirus infectious of mouse testes. J Virol, 1986, 57: 1149–1154PubMedGoogle Scholar
  14. 14.
    Kapranos N, Petralcon E, Anastasiadou Cet al. Detection of herps simplex virus, cytomegalovirus, and Epstein-barr virus in the semen of men attending an infertility clinic. Fertil Steril, 2003, 79: 1566–1570PubMedCrossRefGoogle Scholar
  15. 15.
    Xiong C L, Dong L Y, Su Jet al. Effect of creatine phosphate on sperm motility and viability after being defrosted. Chin J Androl (Chinese), 1998, 12: 147–149Google Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  • Xiong Jinwen
    • 1
  • Xiong Chengliang
    • 1
  • Tian Yonghong
    • 1
  • Hu Lian
    • 1
  • Wei Hai
    • 1
  1. 1.Center of Reproductive Medicine, Research Institute of Family Planning, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina

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