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Structural characterization of the Sry 5’ regulatory region of bovine

  • Shi Hui-fang
  • Cheng Han-hua
  • Guo Yi-qing
  • Liu Li
  • Yu Qi-xing
  • Zhou Rong-jia
Abstract

Abstract

By amplification of the polymerase chain reaction (PCR), the two fragments of 680 bp and 640 bp of 5’ regulatory region of Chinese bovine Sry gene were subcloned into pUC18. Sequence analysis shows that the fragment of total 1040 bp, which is highly conserved within bovine species, contains a start code ATG and a core promoter.

Key words

sex determination bovine promoter evolution 

Copyright information

© Springer 2000

Authors and Affiliations

  • Shi Hui-fang
    • 1
  • Cheng Han-hua
    • 1
  • Guo Yi-qing
    • 1
  • Liu Li
    • 1
  • Yu Qi-xing
    • 1
  • Zhou Rong-jia
    • 1
  1. 1.College of Life SciencesWuhan UniversityWuhanChina

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