Advertisement

Chromosome G-banding in situ hybridization of RFLP marker umc58 linked with the gene hm1 dictatingHelminthosporium carbonum susceptibility1 in maize

  • Song Yunchun
  • Ren Nan
  • Mao Ninghui
  • Liu Lihua
Article

Abstract

The technique of simultaneous G-banding and in situ hybridization has been developed in plants for the first time. Using this technique, RFLP marker umc58 closely linked with the hm1 gene dictatingHelminthosporium carbonum susceptibility1 was localized onto 1L3 (chromosome 1, long arm, the third band from the centromere to the end of the arm), 5L5 and 9L5. The results demonstrated that umc58 was a triplicated sequence. It was deduced that umc58 probably was in a duplicated region that includes a part ofHelminthosporium carbonum susceptibility genes (hm1 and hm2), as the hybridization sites of umc58 in chromosomes 1 and 9 were those at which the genes localize. The techniques of simultaneous G-banding and ISH in plants are discussed.

Key words

In situ hybridization chromosome G-banding maize RFLP markers 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Jiang J, Gill B S. Sequential chromosome banding and in situ hybridization analysis.Genome, 1993,36: 792–795CrossRefGoogle Scholar
  2. 2.
    Drewry A. G-banded chromosomes inPinus resinosa.J Hered, 1982,73: 305–306Google Scholar
  3. 3.
    Song Y C, Liu L H, Ding Y,et al. Comparisons of G-banding patterns in six species of the Poaceae.Hereditas, 1994,121: 31–38CrossRefGoogle Scholar
  4. 4.
    Richard F, Vogt N, Muleris M,et al. Increased FISH efficiency using APC probes genetrated by direct incorporation of labeled nucleotides by PCR.Cytogenet Cell Genet, 1994,65: 169–171Google Scholar
  5. 5.
    Ambros P F, Matzke M A, Matzke A J M. Detection of a 17 kb unique sequence (T-DNA) in plant chromosomes by in situ hybridization.Chromosoma, 1986,94: 11–18CrossRefGoogle Scholar
  6. 6.
    Ren N, Song Y C, Bi X Z,et al. The physical location of the maize cdc2 gene by in situ hybridization.J Wuhan Univ (Natural Science Edition), 1996,42 (6): 740–746 (in Chinese)Google Scholar
  7. 7.
    Gustafson J P, Dille' J E. The chromosome location ofOryza sativa recombination linkage groups.Proc Natl Acad Sci U S A, 1992,89: 8646–8650CrossRefGoogle Scholar
  8. 8.
    Song Y C, Gustafson J P. The physical location of fourteen RFLP markers in rice (Oryza sativa L).Theor Appl Genet, 1995,90: 113–119Google Scholar
  9. 9.
    Coe E H. Gene list and working maps.Maize Genet. Coop Newsl, 1993,67: 133–168Google Scholar
  10. 10.
    Dowty J, Helentjaris T. Duplicated RFLP loci are abundant in the genome.Maize Genet Coop Newsl, 1992,66: 106Google Scholar
  11. 11.
    Molina M C, Poggio L, Naranjo C A. Cytogenetic analysis of the hybridsZea mays ssp mays × Z mays ssp parviglumis andZ mays ssp mays × Z ssp mexicana.Maize Genet Coop News, 1992,66: 60Google Scholar
  12. 12.
    Heslop-Harrison J S. The molecular cytogenetics of plants.J Cell Sci, 1991,100: 15–21Google Scholar

Copyright information

© Springer 1997

Authors and Affiliations

  • Song Yunchun
    • 1
  • Ren Nan
    • 1
  • Mao Ninghui
    • 1
  • Liu Lihua
    • 1
  1. 1.College of Life SciencesWuhan UniversityWuhanChina

Personalised recommendations