Skip to main content
SpringerLink
Log in

Influence ofPhanerochœte chrysosporium culture conditions on mitochondrial cytochrome content

  • Papers
  • Published:
Folia Microbiologica Aims and scope Submit manuscript

Abstract

UV-Visible diffuse reflectance spectroscopy was applied for determination of mitochondrial cytochromes in whole mycelial pellets of the basidiomycetePhanerochœte chrysosporium. The lignin-peroxidase activity and mitochondrial cytochrome content were measured in pellets of cultures grown on a growth medium with various concentrations of nitrogen and Mn(II), and with or without the addition of Tween 20. In cultures grown under conditions that induce the expression of lignin-peroxidase activity, a decrease of cytochromeaa 3 content was observed at the time of the onset of lignin-peroxidase activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Asther M.G., Corrieu G., Drapron R., Odier E.: Effect of Tween 80 and oleic acid on ligninase production byPhanerochœte chrysosporium INA-12.Enz. Microb. Technol. 9, 245–249 (1987).

    Article  CAS  Google Scholar 

  • Bonnarme P., Delattre M., Drouet H., Corrieu G., Asther M.: Toward a control of lignin and manganese peroxidases hyper secretion byPhanerochœte chrysosporium in agitated vessels: Evidence of the superiority of pneumatic bioreactors on mechanically agitated bioreactors.Biotechnol. Bioeng. 41, 440–450 (1992).

    Article  Google Scholar 

  • Bonnarme P., Jeffries T.W.: Mn(II) regulation of lignin peroxidases and manganese-dependent peroxidases from lignin-degrading white rot fungi.Appl. Environ. Microbiol. 56, 210–217 (1990).

    PubMed  CAS  Google Scholar 

  • Bumpus J.A., Tien M., Wright D., Aust S.D.: Oxidation of persistent environmental pollutants by a white rot fungus.Science 228, 1434–1436 (1985).

    Article  PubMed  CAS  Google Scholar 

  • Jäger A., Croan S., Kirk T.K.: Production of ligninases and degradation of lignin in agitated submerged cultures ofPhanerochœte chrysosporium.Appl. Environ. Microbiol. 50, 1274–1278 (1985).

    PubMed  Google Scholar 

  • Jeffries T.W., Choi S., Kirk T.K.: Nutritional regulation of lignin degradation byPhanerochœte chrysosporium.Appl. Environ. Microbiol. 42, 290–296 (1981).

    PubMed  CAS  Google Scholar 

  • Keyser P., Kirk T.K., Zeikus J.G.: Ligninase enzyme system ofPhanerochœte chrysosporium: Synthesized in the absence of lignin in response to nitrogen starvation.J. Bacteriol. 135, 790–797 (1987).

    Google Scholar 

  • Kirk T.K., Farrell R.L.: Enzymatic “combustion”: The microbial degradation of lignin.Ann. Rev. Microbiol. 41, 465–505 (1987).

    Article  CAS  Google Scholar 

  • Kirk T.K., Schultz E., Connors W.J., Lorenz L.F., Zeikus J.G.: Influence of culture parameters on lignin degradation byPhanerochœte chrysosporium.Arch. Microbiol. 117, 277–285 (1978).

    Article  CAS  Google Scholar 

  • Leštan D., Černilec M., Perdih A.: Determination of lignin-peroxidase activity inP. chrysosporium pellets with diffuse reflectance spectroscopy.Appl. Microbiol. Biotechnol. 38, 570–573 (1993a).

    Article  Google Scholar 

  • Leštan D., Černilec M., Štrancar A., Perdih A.: Influence of some surfactants and related compounds on lignin-peroxidase activity ofPhanerochœte chrysosporium.FEMS Microbiol. Lett. 106, 17–22 (1993b).

    Article  Google Scholar 

  • Leštan D., Leštan M., Perdih A.: Physiological aspects of biosynthesis of lignin peroxidases byPhanerochœte chrysosporium.Appl. Environ. Microbiol. 60, 606–612 (1994).

    PubMed  Google Scholar 

  • Leštan D., Podgornik H., Perdih A.: Analysis of fungal pellets by UV-visible spectrum diffuse reflectance spectroscopy.Appl. Environ. Microbiol. 60, 606–612 (1993c).

    Google Scholar 

  • Leštan D., Štrancar A., Perdih A.: Influence of some oils and surfactants on lignin-peroxidase activity, growth and lipid fatty acids ofPhanerochœte chrysosporium.Appl. Microbiol. Biotechnol. 34, 426–428 (1990).

    Google Scholar 

  • Linko S.: Production and characterization of extracellular lignin peroxidase from immobilizedPhanerochœte chrysosporium in a 10-L bioreactor.Enz. Microb. Technol. 10, 410–417 (1988).

    Article  CAS  Google Scholar 

  • Masaphy S., Lavenon D., Heins Y., Venkateswarlu K., Kelly S.L.: Evidence for cytochrome P-450 and P-450-mediated benzo[a]pyrene hydroxylation in the white rot fungusPhanerochœte chrysosporium.FEMS Microbiol. Lett. 135, 51–55 (1996).

    Article  PubMed  CAS  Google Scholar 

  • Mileski G.J., Bumpus J.A., Jurek M.A., Aust S.D.: Biodegradation of pentachlorophenol by the white rot fungusPhanerochœte chrysosporium.Appl. Environ. Microbiol. 54, 2885–2889 (1988).

    PubMed  CAS  Google Scholar 

  • Tien M., Kirk T.K.: Lignin degrading enzyme fromPhanerochœte chrysosporium: purification, characterisation and catabolytic properties of unique H2O2-requiring oxygenase.Proc. Nat. Acad. Sci. USA. 81, 2280–2284 (1984).

    Article  PubMed  CAS  Google Scholar 

  • Williams J.N.: A method for simultaneous quantitative estimation of cytochromesa,b,c 1 andc in mitochondria.Arch. Biochem. Biophys. 107, 537–543 (1964).

    Article  PubMed  CAS  Google Scholar 

  • Yadav J.S., Quensen IIIJ.F., Tiedje J.M., Reddy C.A.: Degradation of polychlorinated biphenyl mixtures (Aroclors 1242, 1254, and 1260) by the white rot fungusPhanerochœte chrysosporium as evidenced by congener-specific analysis.Appl. environ. Microbiol. 61, 2560–2565 (1995).

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Center for Soil and Environmental Science, Department of Agronomy, University of Ljubjana, 61111, Ljubljana, Slovenia

    D. Leštan

  2. Department of Chemical Technology, University of Ljubljana, 61001, Ljubljana, Slovenia

    A. Perdih

Authors
  1. D. Leštan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Perdih
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Leštan, D., Perdih, A. Influence ofPhanerochœte chrysosporium culture conditions on mitochondrial cytochrome content. Folia Microbiol 42, 473–476 (1997). https://doi.org/10.1007/BF02826556

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02826556

Keywords

Search

Navigation