Folia Microbiologica

, Volume 46, Issue 1, pp 71–72 | Cite as

Restriction and modification systems of ruminal bacteria

  • P. Pristaš
  • V. Molnárová
  • P. Javorský
2nd ISAM (International Symposium on Anaerobic Microbiology) Anaerobic Microbiology: Centeral European Perspective held in Prague (Czechia) November 10–11, 2000


A high frequency of type II restriction endonuclease activities was detected inSelenomonas ruminantium but not in other rumen bacteria tested. Eight different restriction endonucleases were characterized in 17 strains coming from genetically homogeneous local population. Chromosomal DNA isolated fromS. ruminantium strains was found to be refractory to cleavage by various restriction enzymes, implying the presence of methylase activities additional to those required for protection against the cellular endonucleases. The presence of Dam methylation was detected inS. ruminantium strains as well as in several other species belonging to theSporomusa subbranch of low G+C Gram-positive bacteria (Megasphaera elsdenii, Mitsuokella multiacidus).


Modification System Fallow Deer Rumen Bacterium Ruminal Bacterium Methylase Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Hemavathy K.C., Nagaraja V.: DNA methylation in mycobacteria: absence of methylation at GATC (Dam) and CCA/TGG (Dcm) sequences.FEMS Immunol. Med. Microbiol.11, 291–296 (1995).PubMedCrossRefGoogle Scholar
  2. Maniatis T., Fritsch E.F., Sambrook J.:Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1982.Google Scholar
  3. Marmur J.: A procedure for the isolation of deoxyribonucleic acid from microorganisms.J. Mol. Biol.3, 208–218 (1961).CrossRefGoogle Scholar
  4. Molnárová V., Javorský P. Pristaš P.: Prevalence of CTGCAG recognizing restriction and modification systems in ruminal selenomonades.Anaerobe5, 37–41 (1999).PubMedCrossRefGoogle Scholar
  5. Noyer-Wiedner M., Trautner T.A.: Methylation of DNA in prokaryotes, pp. 39–108 in J.P. Jost, H.P. Saluz (Eds):DNA Methylation: Molecular Biology and Biological Significance. Birkhauser Verlag, Basle 1993.Google Scholar
  6. Pristaš P., Molnárová V., Javorský P.: Detection ofN6-methyladenine in GATC sequences ofSelenomonas ruminantium.J. Basic Microbiol.38, 283–287 (1998).PubMedCrossRefGoogle Scholar
  7. Pristaš P., Vanat I., Javorský P.: Variability of endonucleolytic activity indicates high genetic diversity among natural populations ofSelenomonas ruminantium.Folia Microbiol.42, 121–124 (1997).CrossRefGoogle Scholar
  8. Štyriak I., Pristaš P., Javorský P.: Lack of surface receptors not restriction-modification system determines F4 phage resistance inStreptococcus bovis II/1.Folia Microbiol.44, 35–38 (1998).CrossRefGoogle Scholar
  9. Wilson G.G., Murray N.E.: Restriction and modification systems.Ann. Rev. Genet.25, 585–627 (1991).PubMedCrossRefGoogle Scholar

Copyright information

© Folia Microbiologia 2001

Authors and Affiliations

  • P. Pristaš
    • 1
  • V. Molnárová
    • 1
  • P. Javorský
    • 1
  1. 1.Institute of Animal PhysiologySlovak Academy of SciencesKošiceSlovakia

Personalised recommendations