Extracellular non-specific nucleases were observed in some strains belonging to the ruminal species of the genusPrevotella, mostlyP. brevis andP. bryantii. The nuclease fromP. bryantii appeared to be extracellular; it mediates the degradation of the supercoiled plasmid DNAvia an open circle intermediate. The cleavage is not site specific although a preference for certain cleavage sites does seem to exist. Our attempts to clone the wild-typeP. bryantii B14 nuclease inE. coli strain ER1992 that reports on the DNA damage sustained, were unsuccessful probably due to excessive intracellular nuclease activity that killed the cells bearing the gene for the nuclease. On the other hand, the nuclease from a related strain TC1-1, which has a less active enzyme of the same type, was successfully cloned.
Rumen Bacterium Ruminal Bacterium Prevotella Species Nucleolytic Activity Deoxyribonuclease Activity
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