Folia Microbiologica

, Volume 44, Issue 6, pp 669–672 | Cite as

Biodegradability of lignin—Polypropylene composite films

  • M. Mikulášová
  • B. Košíková


Biological degradation of composite lignin-polypropylene films containing 4% organocell lignin was confirmed by treatment with lignin-degrading enzymes produced by the white-rot fungusPhanerochœte chrysosporium. The kinetics ofP. chrysosporium culture in the presence of lignin-containing and lignin-free polypropylene films show that the fungus produced lignin-degrading enzymes into the liquid medium during incubation with the lignin-polypropylene film. The degree of biodegradation of both types of film was followed by monitoring their mechanical properties. Correlation was found between the decrease of elongation at break and the amount of released lignin fragments into the extracellular fluid in the course of microbial treatment. The incorporation of lignin into polyolefins represents a new way of using wastes from pulp and paper industry to reduce the environmental impact factor of waste plastics.


Waste Plastic Isotactic Polypropylene Veratryl Alcohol Biodegradable Plastic Crude Enzyme Preparation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Glasser W.G., Barnett C.A., Muller P.C., Sarkanen K.V.: The chemistry of several novel bioconversion lignins.J. Agric. Food Chem. 31, 921–930 (1983).CrossRefGoogle Scholar
  2. Huang J.C., Shetty A.S., Wang M.S.: Biodegradable plastics: a review.Adv. Polym. Technol. 10, 1023–1030 (1990).CrossRefGoogle Scholar
  3. Kirk T.K., Farrel R.L.: Enzymatic “combustion”: the microbial degradation of lignin.Ann. Rev. Microbiol. 41, 465–505 (1987).CrossRefGoogle Scholar
  4. Kirk T.K., Schultz E., Connors W.J., Lorenz L.F., Zeikus J.G.: Influence of culture parameters on lignin metabolism byPhanerochœte chrysosporium.Arch. Microbiol. 117, 277–285 (1978).CrossRefGoogle Scholar
  5. Košíková B., Demianová V., Kačuráková M.: Sulfur-free lignins as composities of polypropylene films.J. Appl. Polym. Sci. 47, 1065–1073 (1993a).CrossRefGoogle Scholar
  6. Košíková B., Miklešová K., Demianová V.: Characteristics of free radicals in composite lignin—polypropylene films studied by the EPR method.Eur. Polym. J. 29, 1495–1497 (1993b).CrossRefGoogle Scholar
  7. Košíková B., Kačuráková M., Demianová V.: Photooxidation of the composite lignin—polypropylene films.Chem. Papers 47, 132–136 (1993c).Google Scholar
  8. Kratzl K., Claus P., Lonsky W., Gratzl J.S.: Model studies on reactions occurring in oxidations of lignin with molecular oxygen in alkaline media.Wood Sci. Technol. 8, 35–49 (1974).Google Scholar
  9. Kuwahara M., Glenn J.K., Morgan M.A., Gold M.H.: Separation and characterization of two extracellular H2O2-dependent oxidases from ligninolytic cultures ofPhanerochœte chrysosporium.FEBS Lett. 169, 247–250 (1984).CrossRefGoogle Scholar
  10. Lee B., Pometto III.A.L., Fratzke A., Bailey T.B. Jr.: Biodegradation of degradable plastic polyethylene byPhanerochœte chrysosporium andStreptomyces species.Appl. Environ. Microbiol. 57, 678–685 (1991).PubMedGoogle Scholar
  11. Miller G.L.: Dinitrosalicylic acid reagent for determination of reducing sugars.Anal. Chem. 31, 426–428 (1959).CrossRefGoogle Scholar
  12. Milstein O., Gersonde R., Huttermann A., Chen M., Meister J.J.: Fungal biodegradation of lignopolystyrene graft copolymers.Appl. Environ. Microbiol. 58, 3225–3232 (1992).PubMedGoogle Scholar
  13. Tien M.: Properties of ligninase fromPhanerochœte chrysosporium and their posible applications.Crit. Rev. Microbiol. 15, 141–168 (1987).PubMedCrossRefGoogle Scholar
  14. Tien M., Kirk T.K.: Lignin-degrading enzyme fromPhanerochœte chrysosporium. Purification, characterization and catalytic properties of a unique H2O2-requiring oxygenase.Proc. Nat. Acad. Sci. USA 81, 2280–2284 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1999

Authors and Affiliations

  • M. Mikulášová
    • 1
  • B. Košíková
    • 2
  1. 1.Department of Biochemistry and Microbiology, Faculty of Chemical TechnologySlovak University of TechnologyBratislavaSlovakia
  2. 2.Institute of ChemistrySlovak Academy of ScienceBratislavaSlovakia

Personalised recommendations