Plant Molecular Biology Reporter

, Volume 18, Issue 1, pp 51–59 | Cite as

Integrated bombardment andAgrobacterium transformation system: An alternative method for soybean transformation

  • Annette Droste
  • Giancarlo Pasquali
  • Maria Helena Bodanese-Zanettini


Described here is a new method for soybean transformation, based on microwounding of embryonic clumps by particle bombardment prior to inoculation with anAgrobacterium suspension. The method combines the advantages of somatic embryogenesis and gene transfer through an integrated transformation system. This is the first report of application of this technique to transformation ofGlycine max.

Key words

Agrobacterium Glycine max particle bombardment somatic embryogenesis transformation 



2,4-dichlorophenoxyacetic acid


gene for hygromicin phosphotransferase


gene for β-glucuronidase with an intron


optic density

Tween 20



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Copyright information

© Springer 2000

Authors and Affiliations

  • Annette Droste
    • 1
  • Giancarlo Pasquali
    • 2
  • Maria Helena Bodanese-Zanettini
    • 3
  1. 1.Centro de Ciências da SaúdeUniversidade do Vale do Rio dos SinosSão LeopoldoBrazil
  2. 2.Centro de Biotecnologia do Estado do Rio Grande do Sul e Departamento de Biotecnologia, Instituto de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  3. 3.Departamento de Genética, Instituto de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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