Molecular Biotechnology

, Volume 2, Issue 2, pp 129–134 | Cite as

Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogenes

  • Boguslaw Szewczyk
  • Donald F. Summers


Following separation of proteins by SDS-PAGE, they are electroblotted onto polyvinylidene difluoride membranes (Immobilon). Protein bands of interest are excised, and the proteins are eluted from the membrane with detergent-containing buffers at pH 9.5. The method routinely yields recovery of 70–90%, and this is independent of protein molecular weight.

Index Entries

Polyvinylidine difluoride protein blotting immunogens Immobilon SDS-PAGE thioredoxin renaturation 


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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Boguslaw Szewczyk
    • 1
  • Donald F. Summers
    • 1
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of California, College of MedicineIrvine

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