Efficient elution of purified proteins from polyvinylidene difluoride membranes (immobilon) after transfer from SDS-PAGE and their use as immunogenes
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Following separation of proteins by SDS-PAGE, they are electroblotted onto polyvinylidene difluoride membranes (Immobilon). Protein bands of interest are excised, and the proteins are eluted from the membrane with detergent-containing buffers at pH 9.5. The method routinely yields recovery of 70–90%, and this is independent of protein molecular weight.
Index EntriesPolyvinylidine difluoride protein blotting immunogens Immobilon SDS-PAGE thioredoxin renaturation
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