Somatic embryogenesis in plantain banana
- 209 Downloads
A cell suspension of French Sombre plantain banana (Musa spp. AAB genome) was initiated from callus obtained from young male flowers. Histological examination enabled us to describe and follow the evolution of the suspension consisting of: embryogenic aggregates, proembryos, nodules, and isolated cells. It demonstrated the unicellular origin of somatic embryos, either during maintenance of the suspension or after plating on a semisolid medium. The cells from which the embryos originated had no starch but only protein reserves. Plating 1 ml of packed cells from the suspension led to the formation of 105 embryos of which 10 to 40% could be converted into plantlets.
Key wordsin vitro histology cell suspension embryogenic cell
Unable to display preview. Download preview PDF.
- Dhed'a, D.; Dumortier, F.; Panis, B., et al. Plant regeneration in cell suspension cultures of cooking banana cv. “Bluggoe” (Musa spp. ABB group). Fruits 46:125–135; 1991.Google Scholar
- Emons, A. M. C. Somatic embryogenesis: cell biological aspects. Acta Bot. Neerl. 43:1–14; 1994.Google Scholar
- Ma, S. S. Somatic embryogenesis and plant regeneration from cell suspension culture of banana. Proceedings of symposium on tissue culture of horticultural crops. Taipei, Taiwan 1988 March 8–9. Published by Department of Horticulture, National Taiwan University. June 1991:181–188.Google Scholar
- Schenk, R. U.; Hildebrandt, A. C. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50:199–204; 1972.Google Scholar
- Street, H. E.; Withers, L. A. The anatomy of embryogenesis in culture. In: Street, H. E., ed. Tissue culture and plant science. London: Academic Press; 1974:71–100.Google Scholar
- Williams, E. G.; Maheswaran, G. Somatic embryogenesis: factors influencing coordinated behaviour of cells as an embryogenic group. Ann. Bot. 57:443–462; 1986.Google Scholar