In Vitro - Plant

, Volume 32, Issue 2, pp 66–71 | Cite as

Somatic embryogenesis in plantain banana

  • A. Grapin
  • J. Schwendiman
  • C. Teisson
Developmental Biology/Morphogenesis


A cell suspension of French Sombre plantain banana (Musa spp. AAB genome) was initiated from callus obtained from young male flowers. Histological examination enabled us to describe and follow the evolution of the suspension consisting of: embryogenic aggregates, proembryos, nodules, and isolated cells. It demonstrated the unicellular origin of somatic embryos, either during maintenance of the suspension or after plating on a semisolid medium. The cells from which the embryos originated had no starch but only protein reserves. Plating 1 ml of packed cells from the suspension led to the formation of 105 embryos of which 10 to 40% could be converted into plantlets.

Key words

in vitro histology cell suspension embryogenic cell 


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Copyright information

© Society for In Vitro Biology 1996

Authors and Affiliations

  • A. Grapin
    • 1
  • J. Schwendiman
    • 1
  • C. Teisson
    • 1
  1. 1.Laboratoire de Culture in vitroUnité de recherche BIOTROP du CIRAD-GERDATMontpellier Cédex 01France

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