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Studies on stomatal function, epicuticular wax and stem-root transition region of polyethylene glycol-treated and nontreatedin vitro grape plantlets

  • Massoma Ali-Ahmad
  • Harrison G. Hughes
  • Farida Safadi
Cell Biology

Summary

Scanning electron microscopy, light microscopy, and gravimetric analysis was used to evaluate stomatal function, epicuticular wax, and the stem-root transition region of grape (Vitis sp. ‘Valiant’) plantlets grownin vitro, polyethylene glycoltreatedin vitro, and greenhouse-grown plants. Scanning electron microscopic studies of leaf surfaces ofin vitro-grown plants showed widely open stomata as compared to leaf stomata of polyethylene glycol-treatedin vitro-cultured and greenhouse-grown plants. Ultrastructurally, leaf epicuticular wax ofin vitro plants was less dense than in their polyethylene-treated and greenhouse counterparts. Quantitatively,in vitro-grown plants had reduced epicuticular was as compared to polyethylene glycol-treated and greenhouse-grown plants. Light microscopic studies showed no obvious differences in the vascular connections in the stem-root transition region ofin vitro-cultured, polyethylene glycol-treatedin vitro-cultured, and greenhouse-grown plants. It is therefore likely that the rapid wilting and desiccation observed after transplantingin vitro grape plantlets is due to their defective stomatal function and reduced epicuticular wax and may not be due to poor water transport associated with vascular connection.

Key words

acclimatization grape Vitis sp. ‘Valiant’ in vitro micropropagation epicuticular wax 

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Copyright information

© Society for In Vitro Biology 1998

Authors and Affiliations

  • Massoma Ali-Ahmad
    • 1
  • Harrison G. Hughes
    • 2
  • Farida Safadi
    • 3
  1. 1.Plant Biotechnology Laboratory, Division of Agricultural SciencesFlorida A & M UniversityTallahassee
  2. 2.Department of Horticulture and Landscape ArchitectureColorado State UniversityFort Collins
  3. 3.Department of BiologyColorado State UniversityFort Collins

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