Micropropagation ofUraria picta, a medicinal plant, through axillary bud culture and callus regeneration
- 221 Downloads
Micropropagation ofUraria picta, a leguminous herb, was achieved through axillary bud culture and nodal callus culture. Bud break was best when nodes were cultured on Murashige and Skoog (1962) (MS) medium supplemented with 2.6 μM α-naphthalene acetic acid and 4.4 μM N6-benzyladenine. Optimum shoot multiplication was observed in adenine sulphate at 2.47 μM concentration. Competent callus was initiated around the nodal ring of the explant on the basal medium supplemented with cytokinins and auxin (α-naphthalene acetic acid and N6-benzyladenine), which regenerated into new profusely growing shoots on transferring to 0.13 μM N6-benzyladenine. Shoots elongated to 5 node length with 1.11 μM N6-benzyladenine were rooted on half-strength MS basal medium. The rooted plants were successfully established with 80% survival. About 400 such plants were transferred to the field.
Key wordsbioprospecting conservation organogenesis plant-tissue culture
Unable to display preview. Download preview PDF.
- Allen, O. N.; Allen, K. E. The leguminosae. Madison, WI: University of Wisconsin Press; 1981:672–673.Google Scholar
- Anonymous. In: The wealth of India. Raw materials Vol. 10 New Delhi. Publication and Information Directorate, CSIR; 1976:443.Google Scholar
- Bajaj, Y.P.S. Biotechnology in agriculture and forestry. Vol. 1. Trees. Berlin, Germany: Springer-Verlag; 1986.Google Scholar
- Bressan, P. H.; Kirh, Y. J.; Hyndman, S. E., et al. Factors affecting in vitro propagation of rose. J. Am. Soc. Hortic. Sci. 107:979–990; 1982.Google Scholar
- Feinsilver, J. M. In: Zakri, A. H., ed. Biodiversity prospecting: prospects and realities. Genetics Society of Malaysia. Universiti Kebangsaan, Selangor, Malaysia; 1995:21–58.Google Scholar
- Jain, S. K.; Defilipps, R. A. Medical plants of India. Vol. 1. Alganao, MI: Reference Publication; 1991:342.Google Scholar
- Kartha, K. K. In: Kartha, K. K. ed. Cryopreservation of plant cells and organs. Boca Raton, FL: CRC Press; 1985:115–134.Google Scholar
- Kartha, K. K.; Pahl, K.; Leung, N. L., et al. Plant regeneration from meristems of grain legumes: soybean, cowpea, peanut, chickpea and bean. Can. J. Bot. 59:1671–1679; 1981.Google Scholar