Skip to main content
SpringerLink
Log in

Influence of some plant growth regulators on the growth and organogenesis ofCymbidium aloifolium (L.) Sw. seed-derived rhizomesin vitro

  • Developmental Biology/Morphogenesis
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

An efficient procedure is outlined forin vitro regeneration of an epiphytic orchid,Cymbidium aloifolium (L.) Sw. using rhizomes developed from seeds. Murashige and Skoog's (1962) medium (MS) containing indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or 1-naphthaleneacetic acid (NAA) stimulated growth and proliferation of rhizomes with NAA being most effective at 5.0 mg.l−1 (27.0 μM). Shoot bud differentiation was induced in the apical portions of the rhizomes on MS medium containing kinetin (Kn) or N6-benzyladenine (BA). The highest frequency of shoot regeneration (91.5%) and the maximum number of shoot buds formed (3.5 shoots/rhizome) were recorded with BA at 1.0 mg.l−1 (4.4 μM). NAA (0.1 mg.l−1, 0.54 μM), whenever added to the medium in conjunction with BA (1.0 mg.l−1, 4.4 μM), slightly enhanced the frequency of shoot bud regeneration (92.6%) and the number of shoot buds formed (5.2 shoots/rhizome). Moreover, an NAA-BA combination induced rooting in regenerated shoots thereby producing complete plantlets in one step. Shoots developed on cytokinin-supplemented medium were rooted on MS containing NAA at 1.0 mg.l−1 (5.4 μM). Regenerated plantlets were acclimated and eventually established in a garden.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arditti, J.; Ernst R Micropropagation of orchids, New York: John Wiley and Sons; 1993.

    Google Scholar 

  • Begum, A. A.; Tamaki, M.; Tahara, M., et al. Somatic embryogenesis inCymbidium throughin vitro culture of inner tissue of protocorm like bodies. J. Jpn. Soc. Hortic. Sci. 63(2):419–427; 1994a.

    CAS  Google Scholar 

  • Begum, A. A.; Tamaki, M.; Kako, S. Formation of protocorm like bodies (PLB) and shoot development throughin vitro culture of outer tissue ofCymbidium PLB. J. Jpn. Soc. Hortic. Sci. 63(3):663–673; 1994b.

    Article  CAS  Google Scholar 

  • Chung, J. D.; Chun, C. K.; Choi, S. O. Asymbiotic germination ofCymbidium ensifolium II. Effects of several supplements to the medium, pH values, light and/or dark culture periods on growth of rhizome and organogenesis from rhizomes. J. Korean Soc. Hortic. Sci. 26:186–192; 1985.

    CAS  Google Scholar 

  • Hasegawa, A.; Ohashi, H.; Goi, M. Effects of BA on rhizome length, mechanical treatment and liquid shaking cultures on the shoot formation from rhizomes inCymbidium faberi Rolfe. Acta Hortic. 166:25–40; 1985.

    Google Scholar 

  • Lee, J. S. Effect of NAA, BA and temperature on growth and shoot differentiation ofCymbidium niveo-marginatum Makino (OrientalCymbidium) rhizomein vitro. Cheju National University J. 27:21–27; 1988.

    CAS  Google Scholar 

  • Lee, J. S.; So, I. S. Effect of NAA and BA on dark culture ofCymbidium virescences rhizomein vitro. Subtrop. Agric. Cheju National University 2:133–139; 1985.

    Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962.

    Article  CAS  Google Scholar 

  • Paek, K. Y.; Yeung, E. C. The effects of 1-naphthaleneacetic acid and N6-benzyladenine on the growth ofCymbidium forrestii rhizomesin vitro. Plant Cell Tissue Organ Cult. 24:65–71; 1991.

    Article  CAS  Google Scholar 

  • Prakash, L.; Lee, C. L.; Loh, C. S., et al.In vitro propagation of commercial orchids: an assessment of current methodology and development of a novel approach—thin cross-section culture. In: Islam, A. S., ed. Plant tissue culture, New Delhi, India: Oxford & IBH Publishing Co. Pvt. Ltd.; 1996:42–49.

    Google Scholar 

  • Shimasaki, K.; Uemoto, S. Micropropagation of a terrestrialCymbidium species using rhizomes developed from seeds and pseudobulbs. Plant Cell Tissue Organ Cult. 22:237–244; 1990.

    Article  CAS  Google Scholar 

  • Shimasaki, K.; Uemoto, S. Rhizome induction and plantlet regeneration ofCymbidium goeringii from flower bud culturesin vitro. Plant Cell Tissue Organ Cult. 25:49–52; 1991.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Cytogenetics, Post-Graduate Department of Botany, Utkal University, 751 004, Bhubaneswar, Orissa, India

    N. R. Nayak, P. K. Chand, S. P. Rath & S. N. Patnaik

Authors
  1. N. R. Nayak
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. K. Chand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. P. Rath
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. N. Patnaik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nayak, N.R., Chand, P.K., Rath, S.P. et al. Influence of some plant growth regulators on the growth and organogenesis ofCymbidium aloifolium (L.) Sw. seed-derived rhizomesin vitro . In Vitro Cell.Dev.Biol.-Plant 34, 185–188 (1998). https://doi.org/10.1007/BF02822706

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02822706

Key words

Search

Navigation