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Folia Microbiologica

, Volume 34, Issue 3, pp 238–242 | Cite as

Scanning electron microscope study ofTorulopsis ethanolitolerans prepared by glutaraldehyde—Thiosemicarbohydrazide procedure

  • D. Hulínská
  • H. Mohelská
  • L. Adámek
  • M. Rut
Article
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Abstract

Torulopsis ethanolitolerans subject to both the sparing and coarse heat treatment were studied in the scanning electron microscope. The reduction of adhesivity, increased permeability and higher rigidity of the yeast wall was achieved by an original glutaraldehyde—paraformaldehyde fixation, low osmolarity in vacuo and subsequent thiosemicarbohydrazide incubation, followed by addition of metal salt. The impregnation of the metal throughout the specimen due to the reaction of the thiosemicarbohydrazide with glutaraldehyde allowed viewing of small or intricate surface details of the yeast. Structural differences of the yeast processed by sparing and coarse heat treatment were shown to be better from the thiosemicarbohydrazide incubated samples compared to those that were prepared with osmium tetroxide.

Keywords

Osmium Tetroxide Sodium Cacodylate Buffer Scanning Electron Microscopy Specimen Galloylglucoses Membrane Blister 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© ACADEMIA, Publishing House of the Czechoslovak Academy of Sciences 1989

Authors and Affiliations

  • D. Hulínská
    • 1
  • H. Mohelská
    • 1
  • L. Adámek
    • 2
  • M. Rut
    • 2
  1. 1.Institute of Hygiene and EpidemiologyPrague
  2. 2.Research Institute of Food IndustryPrague

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