Folia Microbiologica

, Volume 35, Issue 4, pp 363–367 | Cite as

Membrane biosensor for the determination of iron (II, III) based on immobilized cells ofThiobacillus ferrooxidans

  • M. Mandl
  • L. Macholán
Brief Report


The bacterial electrode for amperometric determination of iron ions is based on a Clark-type oxygen electrode, on the measuring part of which a paste containing a mixture of jarosite precipitate and iron-oxidizing bacteria is mounted with the aid of a cellophane membrane. In acidic media a biochemical oxidation of Fe2+ connected with oxygen consumption takes place in the biocatalytic layer. Fe3+ is determined after its reduction to Fe2+. The determination limit is 60 μmol/L, the stability of the electrode is two months at room temperature.


Jarosite Determination Limit Total Iron Content Thiobacillus Ferrooxidans Cellophane Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. DiSpirito A.A., Tuovinen O.H.: Oxidations of nonferrous metals by thiobacilli, pp. 11–29 inMicrobial Chemoautotrophy (W.R. Strohl, O.H. Tuovinen, Eds.). Ohio State University Press, Columbus 1984.Google Scholar
  2. Grishin S.I., Tuovinen O.H.: Scanning electron microscopic examination ofThiobacillus ferrooxidans on different support matrix materials in packed bed and fluidized bed bioreactors.Appl. Microbiol. Biotechnol. 31, 505–511 (1989).CrossRefGoogle Scholar
  3. Holzbecher Z., Jenik J., Sůcha L., Vlácil F., Vrbský J.:Analytical Chemistry, p. 312 (in Czech). SNTL, Prague 1968.Google Scholar
  4. Ingledew J.W.:Thiobacillus ferrooxidans: the bioenergetics of an acidophilic chemolithotroph.Biochim. Biophys. Acta 683, 89–117 (1982).CrossRefPubMedGoogle Scholar
  5. Lazaroff N., Melanson L., Lewis E., Santoro N., Pueschel C.: Scanning electron microscopy and infrared spectroscopy of iron sediments formed byThiobacillus ferrooxidans.Geomicrobiol. J. 4, 231–268 (1985).CrossRefGoogle Scholar
  6. Macholán L.: Biocatalytic membrane electrodes, inBiosensors and Bioinstrumentation (D.L. Wise, Ed.). M. Dekker, New York (in press) 1990.Google Scholar
  7. Mandl M., Dočekalová H.: Use of the respiration activity ofThiobacillus ferrooxidans for the specific determination of iron (II,III).Folia Microbiol 30, 105–109 (1985).CrossRefGoogle Scholar
  8. Silverman M.P., Lundgren D.G.: Studies on the chemoautotrophic iron bacteriumFerrobacillus ferrooxidans. I. An improved medium and harvesting procedure for securing high cell yields.J. Bacteriol. 77, 642–647 (1959).PubMedPubMedCentralGoogle Scholar

Copyright information

© ACADEMIA, Publishing House of the Czechoslovak Academy of Sciences 1990

Authors and Affiliations

  • M. Mandl
    • 1
  • L. Macholán
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceMasaryk UniversityBrnoCzechoslovakia

Personalised recommendations