Membrane biosensor for the determination of iron (II, III) based on immobilized cells ofThiobacillus ferrooxidans
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The bacterial electrode for amperometric determination of iron ions is based on a Clark-type oxygen electrode, on the measuring part of which a paste containing a mixture of jarosite precipitate and iron-oxidizing bacteria is mounted with the aid of a cellophane membrane. In acidic media a biochemical oxidation of Fe2+ connected with oxygen consumption takes place in the biocatalytic layer. Fe3+ is determined after its reduction to Fe2+. The determination limit is 60 μmol/L, the stability of the electrode is two months at room temperature.
KeywordsJarosite Determination Limit Total Iron Content Thiobacillus Ferrooxidans Cellophane Membrane
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