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Folia Microbiologica

, Volume 35, Issue 4, pp 363–367 | Cite as

Membrane biosensor for the determination of iron (II, III) based on immobilized cells ofThiobacillus ferrooxidans

  • M. Mandl
  • L. Macholán
Brief Report

Abstract

The bacterial electrode for amperometric determination of iron ions is based on a Clark-type oxygen electrode, on the measuring part of which a paste containing a mixture of jarosite precipitate and iron-oxidizing bacteria is mounted with the aid of a cellophane membrane. In acidic media a biochemical oxidation of Fe2+ connected with oxygen consumption takes place in the biocatalytic layer. Fe3+ is determined after its reduction to Fe2+. The determination limit is 60 μmol/L, the stability of the electrode is two months at room temperature.

Keywords

Jarosite Determination Limit Total Iron Content Thiobacillus Ferrooxidans Cellophane Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© ACADEMIA, Publishing House of the Czechoslovak Academy of Sciences 1990

Authors and Affiliations

  • M. Mandl
    • 1
  • L. Macholán
    • 1
  1. 1.Department of Biochemistry, Faculty of ScienceMasaryk UniversityBrnoCzechoslovakia

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