Cell Biophysics

, Volume 26, Issue 1, pp 45–55 | Cite as

The electrokinetic surface of five enteropathogenicEscherichia coli serogroups

  • Adriana Hamond Regua Mangia
  • Lúcia Martins Teixeira
  • Fernando Costa e Silva Filho


The surface ionogenicity of five enteropathogenicEscherichia coli serogroups (O111:H2, O111:H12, O125:H9, O119:H6, and O26:H11) was investigated by electrokinetical approaches. All of the studied surfaces are negatively charged with their mean values of zeta potential (ZP) varying from −9.0 (O26:H11) to −11.9 mV (O111:H2). The populational behavior of the all bacteria are similar since very high ZP values varying from −26 to −30 mV were obtained in experiments carried out with the slip plane calculated at 6.83 nm from the cell surface. All the surfaces are extremely acidic, because the isoelectrophoretic points are localized at pH values below 3.0. Treatment of the microorganisms with neuraminidase did not alter their surface anionogenicity, while treatment with trypsin or phospholipase C reduced their negative charge.

Index Entries

Escherichia coli EPEC cell surface surface charge 


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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Adriana Hamond Regua Mangia
    • 1
    • 2
  • Lúcia Martins Teixeira
    • 3
  • Fernando Costa e Silva Filho
    • 1
  1. 1.Instituto de Biofśicia Carlos Chagas FilhoUFRJ-CCSRio de Janeiro
  2. 2.Escola Nacional de Saúde PúblicaFIOCRUZRio de Janeiro
  3. 3.Instituto de MicrobiologiaUFRJRio de JaneiroBrazil

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