Folia Microbiologica

, Volume 47, Issue 4, pp 359–363 | Cite as

Biological activity of secondary metabolites produced by a strain ofPseudomonas fluorescens



Biological activity of secondary metabolites produced by a plant-growth-promotingPseudomonas fluorescens was evaluated. The strain produced antibiotics phenazine (PHE), 2,4-diacetylphloroglucinol (PHL) and siderophore pyoverdin (PYO) in standard King’s B and succinic acid media, respectively. After extraction, PYO was identified by comparing the UV-spectra and moss-green color development after ‘diazotized sulfanilic acid’ (DSA) spray in TLC. PHE and PHL were identified by comparing standard compounds on TLC and orange-color development immediately after DSA spray.In vitro antibiosis study of the metabolites revealed their antibacterial and antifungal activity against bacterial test organismsCorynebacterium sp.,Mycobacterium phlei andM. smegmatis and test fungiFusarium moniliforme, F. oxysporum, F. semitectum, F. solani andRhizoctonia solani. A statistically significantly higher plant growth was recorded in siderophore-amended plantlets under gnotobiotic conditions whereas PHE and PHL did not show any plant-growth-promoting activity. These results support the importance of the secondary metabolites produced by the strainP. fluorescens in enhancing plant growth and in controling fungal and bacterial pathogens.


Antifungal Activity Tomato Spotted Wilt Virus Cyanic Acid Mycobacterium Smegmatis Fusarium Moniliforme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



4-diazobenzene sulfonic acid (‘diazotized sulfanilic acid’)


King’s B broth


nutrient agar


potato dextrose agar








succinate medium


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  1. Ahl P., Viisard C., Defago G.: Iron bound-siderophores, cyanic acid, and antibiotics involved in suppression ofThielaviopsis basicola by aPseudomonas fluorescens strain.J. Phytopathol. 116, 121–134 (1986).CrossRefGoogle Scholar
  2. Baker R.: Biological control of plant pathogens: definitions, pp. 25–39 in M.A. Hoy, D.C. Herzog (Eds.):Biological Control in Agricultural IPM Systems. Academic Press, New York 1987.Google Scholar
  3. Bakker P.A.H.M., Bakker A.W., Marugg J.D., Weisbeek P.J., Schippers B.: Bioassay for studying the role of siderophores in potato growth stimulation byPseudomonas spp. in short potato rotations.Soil Biol. Biochem. 19, 443–449 (1987).CrossRefGoogle Scholar
  4. Barthakur M., Bezbaruah B.: Plant beneficial effect of two strains ofProteus vulgaris isolated from tea plantations.Indian J. Exp. Biol. 37, 919–924 (1999).PubMedGoogle Scholar
  5. Buysens S., Heugens K., Joseph P., Hofte M.: Involvement of pyochelin and pyoverdin in suppression ofPythium induced damping off tomato byPseudomonas aeruginosa 7NSK2.Appl. Environ. Microbiol. 62, 865–871 (1996).PubMedCentralPubMedGoogle Scholar
  6. Defago G., Haas D.:Pseudomonas as antagonists of soil borne plant pathogens: modes of action and genetic analysis, pp. 249–291 in J.M. Bollag, G. Stotzky (Eds):Soil Biochemistry, Vol. 6. Marcel Dekker, New York 1990.Google Scholar
  7. Deka Boruah H.P., Dileep Kumar B.S.: Plant disease suppression and growth promotion by a fluorescentPseudomonas strain.Folia Microbiol. 47, 137–143 (2002).CrossRefGoogle Scholar
  8. Dileep Kumar B.S.: Fusarial wilt suppression and crop improvement through two rhizobacterial strain in chick pea growing in soil infested withFusarium oxysporum f.sp.ciceris.Biol. Fert. Soils 29, 87–91 (1999).CrossRefGoogle Scholar
  9. Dileep Kumar B.S., Dube H.C.: Seed bacterization with fluorescentPseudomonas for enhanced plant growth, yield and disease control.Soil Biol. Biochem. 24, 539–542 (1992).CrossRefGoogle Scholar
  10. Dileep Kumar B.S., Berggren I., Martensson A.M.: Potential for improving pea production by co-inoculation with fluorescentPseudomonas andRhizobium.Plant & Soil 225, 25–34 (2001).CrossRefGoogle Scholar
  11. Fravel D.R.: Role of antibiosis in the biological control of plant diseases.Ann. Rev. Phytopathol. 26, 75–91 (1988).CrossRefGoogle Scholar
  12. Homma Y., Sato Z., Hirayama F., Konno F., Shiahama H., Suzui T.: Production of antibiotics byPseudomonas cepacia as an agent for biological control of soilborne plant pathogens.Soil Biol. Biochem. 21, 723–728 (1989).CrossRefGoogle Scholar
  13. Homma Y., Suzui T.: Role of antibiotic production in suppression of radish damping-off by seed bacterization withPseudomonas cepacia.Ann. Phytopathol. Soc. Japan 55, 643–652 (1989).CrossRefGoogle Scholar
  14. Howell C.R., Stipanovic R.D.: Control ofRhizoctonia solani on cotton seedlings withPseudomonas fluorescens and with an antibiotic produced by the bacterium.Phytopathology 69, 480–482 (1979).CrossRefGoogle Scholar
  15. Howell C.R., Stipanovic R.D.: Suppression ofPythium ultimum-induced damping-off of cotton seedlings byPseudomonas fluorescens and its antibiotic, pyoluteorin.Phytopathology 70, 715–721 (1980).CrossRefGoogle Scholar
  16. Kandan A., Radja Commare R., Nandakumar R., Ramiah M., Raguchander T., Samiyappan R.: Induction of phenylpropanoid metabolism byPseudomonas fluorescens against tomato spotted wilt virus in tomato.Folia Microbiol. 47, 121–130 (2002).CrossRefGoogle Scholar
  17. Keel C., Wirthner P., Oberhansli T., Voisard C., Burger U., Haas D., Defago G.:Pseudomonas as antagonists of plant pathogens in the rhizosphere: role of the antibiotic 2,4-diacetylphloroglucinol in the suppression of black root rot of tobacco.Symbiosis 9, 327–341 (1990).Google Scholar
  18. Keel C., Schnider U., Maurhofer M., Voisard C., Laville J., Burger U., Philippe W., Haas D., Defago G.: Suppression of root disease byPseudomonas fluorescens CHAO: importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol.Mol. Plant-Microbe Interact. 5, 4–13 (1992).CrossRefGoogle Scholar
  19. King E.O., Ward M.K., Raney D.E.: Two simple media for the demonstration of pyocyanin and fluorescin.J. Lab. Clin. Med. 44, 301–307 (1954).PubMedGoogle Scholar
  20. Kloepper J.W., Schroth M.N., Miller T.D.: Effect of rhizosphere colonization by plant-growth promoting rhizobacteria on potato plant development and yield.Phytopathology 70, 1078–1082 (1980).CrossRefGoogle Scholar
  21. Kloepper J.W., Schroth M.N.: Relationship ofin-vitro antibiosis of plant-growth-promoting rhizobacteria to plant growth and displacement of root microflora.Phytopathology 71, 1020–1024 (1981).CrossRefGoogle Scholar
  22. Kuniho N., Yoshimoto A., Yamada Y.: Promotion of antibiotic production by high ethanol, high NaCl concentration, or heat shock inPseudomonas fluorescens sp. 272.Biol. Biotech. Biochem. 63, 293–297 (1999).CrossRefGoogle Scholar
  23. Lambert B., Leyns F., van Rooyen F., Gossele F., Papon Y., Swings J.: Rhizobacteria of maize and their antifungal activities.Appl. Environ. Microbiol. 53, 1866–1871 (1987).PubMedCentralPubMedGoogle Scholar
  24. Leong J.: Siderophores: their biochemistry and possible role in the biocontrol of plant pathogens.Ann. Rev. Phytopathol. 24, 187–209 (1986).CrossRefGoogle Scholar
  25. Loper J.E.: Role of fluorescent siderophore production in biological control ofPythium ultimum by aPseudomonas fluorescens strain.Phytopathology.78, 166–172 (1988).CrossRefGoogle Scholar
  26. Loper J.E., Buyer J.S.: Siderophores in microbial interactions on plant surfaces.Mol. Plant-Microbe Interact. 4, 5–13 (1991).CrossRefGoogle Scholar
  27. Meyer J.M., Abdallah M.A.: The fluorescent pigment ofPseudomonas fluorescens biosynthesis, purification and physicochemical properties.J. Gen. Microbiol. 107, 319–328 (1978).CrossRefGoogle Scholar
  28. Neilands J.B.: Microbial iron transport compounds (siderophores), pp. 167–202 in G.L. Eichhorn (Ed.):Inorganic Biochemistry, Vol. 1. Elsevier, Amsterdam (The Netherlands) 1979.Google Scholar
  29. Official Methods of Analysis of AOAC International, 16th ed., pp. 27–28. AOAC International, Suite (USA) 1995.Google Scholar
  30. Rosales A.M., Thomashow L., Cook R.J., Mew T.W.: Isolation and identification of antifungal metabolites produced by rice associated antagonisticPseudomonas spp.Phytopathology 85, 1028–1032 (1995).CrossRefGoogle Scholar
  31. Schwyn B., Neilands J.B.: Universal chemical assay for the detection and determination of siderophores.Anal. Biochem. 160, 47–56 (1987).PubMedCrossRefGoogle Scholar
  32. Stutz E., Defago G., Kern H.: Naturally occurring fluorescent pseudomonads involved in suppression of black root rot of tobacco.Phytopathology 76, 181–185 (1986).CrossRefGoogle Scholar
  33. Thomashow L.S., Weller D.M.: Role of phenazine antibiotic fromPseudomonas fluorescens in biological control ofGaeumanomyces graminis var.tritici.J. Bacteriol. 170, 3499–3508 (1988).PubMedCentralPubMedGoogle Scholar
  34. Weller D.M.: Biological control of soil borne plant pathogens in the rhizosphere with bacteria.Ann. Rev. Phytopathol. 26, 351–358 (1988).CrossRefGoogle Scholar
  35. Weller D.M., Cook R.J.: Suppression of take-all of wheat by seed treatment with fluorescent pseudomonads.Phytopathology 73, 463–469 (1983).CrossRefGoogle Scholar
  36. Weller D.M., Thomashow L.S.: Antibiotics: evidence for their production and sites where they are produced, pp. 703–711 in R. Baker, P. Dunn (Eds):New Directions in Biological Control. A.R. Liss, New York 1990.Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2002

Authors and Affiliations

  1. 1.Regional Research LaboratoryCouncil of Scientific and Industrial ResearchJorhatIndia

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