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Folia Microbiologica

, Volume 44, Issue 3, pp 306–310 | Cite as

Production of stress-inducible form of heat-shock protein 70 in mouse peritoneal adherent cells afterin Vivo infection byFrancisella tularensis

  • J. Stulík
  • L. Hernychová
  • A. Macela
  • Z. Kročová
  • M. Kroča
Papers

Abstract

Heat-shock proteins (hsp) are ubiquitously produced molecules which participate in the protection of cells from environmental perturbation. Moreover, the members of the heat-shock protein 60 (hsp60) and 70 (hsp70) families play an important role in pathogen-host interactions. We studiedin vivo production of the 70-kDa heat-shock proteins in the extract of peritoneal exudate cells (PEC) from mice injected intraperitoneally with an attenuated vaccine strain (LVS) ofFrancisella tularensis. We found a differential production of a highly stress-inducible member of the hsp70 family, designated hsp72, in three inbred strains of mice exhibiting either resistance or susceptibility toF. tularensis LVS infection. Whereas in tularemia-resistant mice hsp72 was even expressed in PEC without injection of bacteria and its production further increased on day 3 and slowly declined on days 5 and 7 after injection, in susceptible mice hsp72 production was highly inducble and restricted only to day 3 afterin vivo infection. Further analysis of hsp72 expression revealed intracellular hsp72 accumulation and its preferential production by peritoneal adherent cells.

Keywords

Peritoneal Cell Peritoneal Exudate Cell Francisella Tularensis Live Vaccine Strain Hsp72 Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1999

Authors and Affiliations

  • J. Stulík
    • 1
  • L. Hernychová
    • 1
  • A. Macela
    • 1
  • Z. Kročová
    • 1
  • M. Kroča
    • 1
  1. 1.Institute of Radiobiology and ImmunologyPurkyně Military Medical AcademyHradec KrálovéCzech Republic

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