Folia Microbiologica

, Volume 40, Issue 6, pp 659–664 | Cite as

Characterization of human, mouse and rabbit anti-gliadin antibodies by ELISA and western blotting

  • Z. Vančíková
  • P. Kocna
  • L. Tučková
  • P. Frič
  • M. Dvořák
  • S. Stoyanov
  • H. Tlaskalová-Hogenová


Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, α-, β- and ω-gliadins was found in the patients' sera than in controls.


Celiac Disease Glycopeptide Coeliac Disease Rabbit Antiserum Dermatitis Herpetiformis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



anti-gliadin antibodies


bovine serum albumin


enzyme-linked immunosorbent asay


Frazer's fraction III of gliadin


cœliac disease


glycopeptide of gliadin




monoclonal antibodies


phosphate-buffered saline


peptic-tryptic-pancreatic digest of gliadin


sodium dodecyl sulfate polyacrylamide gel electrophoresis


peroxidase-labeled swine anti-human IgA immunoglobulin fraction


peroxidase-labeled swine-anti-human IgG immunoglobulin fraction


peroxidase-labeled swine anti rabbit immunoglobulins immunoglobulin fraction


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1995

Authors and Affiliations

  • Z. Vančíková
    • 1
  • P. Kocna
    • 2
  • L. Tučková
    • 3
  • P. Frič
    • 2
  • M. Dvořák
    • 2
  • S. Stoyanov
    • 3
  • H. Tlaskalová-Hogenová
    • 3
  1. 1.Department of Clinical Immunology, Faculty Hospital BulovkaPragueCzech Republic
  2. 2.Laboratory of Gastroenterology, First Faculty of MedicineCharles UniversityPragueCzech Republic
  3. 3.Department of Immunology and Gnotobiology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic

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