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Folia Microbiologica

, Volume 40, Issue 6, pp 659–664 | Cite as

Characterization of human, mouse and rabbit anti-gliadin antibodies by ELISA and western blotting

  • Z. Vančíková
  • P. Kocna
  • L. Tučková
  • P. Frič
  • M. Dvořák
  • S. Stoyanov
  • H. Tlaskalová-Hogenová
Papers

Abstract

Monoclonal, hyperimmune rabbit and human serum anti-gliadin antibodies were analyzed by ELISA and immunoblotting techniques. In Western blotting the difference in reactivity between monoclonal and human antibodies was quantitative rather than qualitative. Rabbit antisera differed in reactivity according to the protein used for immunization. The rabbits immunized by the peptic-tryptic pancreatic digest of gliadin reacted similarly to the patients. In ELISA, significantly higher reactivity with crude, A-, glyc-gli, α-, β- and ω-gliadins was found in the patients' sera than in controls.

Keywords

Celiac Disease Glycopeptide Coeliac Disease Rabbit Antiserum Dermatitis Herpetiformis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AGA

anti-gliadin antibodies

BSA

bovine serum albumin

ELISA

enzyme-linked immunosorbent asay

F III

Frazer's fraction III of gliadin

CD

cœliac disease

glyc-gli

glycopeptide of gliadin

Ig

immunoglobulin

mAbs

monoclonal antibodies

PBS

phosphate-buffered saline

PTP

peptic-tryptic-pancreatic digest of gliadin

SDS-PAGE

sodium dodecyl sulfate polyacrylamide gel electrophoresis

SwAHuIgAPx

peroxidase-labeled swine anti-human IgA immunoglobulin fraction

SwAHuIgGPx

peroxidase-labeled swine-anti-human IgG immunoglobulin fraction

SwARPx

peroxidase-labeled swine anti rabbit immunoglobulins immunoglobulin fraction

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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1995

Authors and Affiliations

  • Z. Vančíková
    • 1
  • P. Kocna
    • 2
  • L. Tučková
    • 3
  • P. Frič
    • 2
  • M. Dvořák
    • 2
  • S. Stoyanov
    • 3
  • H. Tlaskalová-Hogenová
    • 3
  1. 1.Department of Clinical Immunology, Faculty Hospital BulovkaPragueCzech Republic
  2. 2.Laboratory of Gastroenterology, First Faculty of MedicineCharles UniversityPragueCzech Republic
  3. 3.Department of Immunology and Gnotobiology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPragueCzech Republic

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