Folia Microbiologica

, Volume 40, Issue 6, pp 615–620 | Cite as

Natural killer cell activity in cœliac disease: Effect ofin vitro treatment on effector lymphocytes and/or target lymphoblastoid, myeloid and epithelial cell lines with gliadin

  • M. A. Farré Castany
  • H. H. Nguyen
  • M. Pospíšil
  • P. Frič
  • H. Tlaskalová-Hogenová


To analyze the possible involvement of natural killer (NK) cell activity in the pathogenetic mechanism of cœliac disease (CD) we measured the spontaneous cytotoxic cell activity of peripheral blood mononuclear cells (PMNC) from patients with CD and from healthy donors. No significant differences were found between the NK cell activity of PMNC from healthy donors and from patients with CD using a standard51Cr release assay. However, a 30-min treatment of PMNC with gliadin inhibited NK cell activity in patients with CD. On the other hand, a 1-d incubation with gliadin induced cytotoxic cell activity of PMNC against the NK-resistant target cells such as the epithelial HT-29 and the lymphoblastoid RAJI cell lines, suggesting that activation of PMNC by cultivation with gliadin can occur.


Healthy Donor Coeliac Disease Natural Killer Cell Receptor Target Cell Line Cytotoxic Cell Activity 



cœliac disease


natural killer


peripheral blood mononuclear cells


Frazer's fraction III


antibody-dependent cell cytotoxicity


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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1995

Authors and Affiliations

  • M. A. Farré Castany
    • 1
  • H. H. Nguyen
    • 1
  • M. Pospíšil
    • 1
  • P. Frič
    • 2
  • H. Tlaskalová-Hogenová
    • 1
  1. 1.Department of Immunology and Gnotobiology, Institute of MicrobiologyAcademy of Sciences of the Czech RepublicPrague 4Czech Republic
  2. 2.Department of Gastroenterology, 1st Faculty of MedicineCharles UniversityPrague 1Czech Republic

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