Abstract
Effects of four lipid peroxidation-inducing pro-oxidants-amphiphilictert-butyl hydroperoxide (TBHP), hydrophobic 1,1′-azobis(4-cyclohexanecarbonitrile) (ACHN), hydrophilic Fe11 and 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH)-on cell growth and on generation of peroxidation products in isolated plasma membrane lipids were determined in four yeast species (S. cerevisiae, S. pombe, R. glutinis andC. albicans) differing in their plasma membrane lipid composition. TBHP and ACHN inhibited cell growth most strongly, Fe11 and AAPH exerted inhibitory action for about 2 h, with subsequent cell growth resumption.S. cerevisiae strain SP4 was doped during growth with unsaturated linoleic (18∶2) and linolenic (18∶3) acids to change its resistance to lipid peroxidation. Its plasma membranes then contained some 30% of these acids as compared with some 1.3% of 18∶2 acid found in undopedS. cerevisiae, while the content of (16∶1) and (18∶1) acids was lower than in undopedS. cerevisiae. The presence of linoleic and linolenic acids inS. cerevisiae cells lowered cell survival and increased the sensitivity to pro-oxidants. Peroxidationgenerated conjugated dienes (CD) were measured in pure TBHP- and ACHN-exposed fatty acids used as standards. The CD level depended on the extent of unsaturation and the pro-oxidant used. The TBHP-induced CD production in a mixture of oleic acid and its ester was somewhat lower than in free acid and ester alone. In lipids isolated from the yeast plasma membranes, the CD production was time-dependent and decreased after a 5–15-min pro-oxidant exposure. ACHN was less active than TBHP. The most oxidizable were lipids fromS. cerevisiae plasma membranes doped with linoleic and linolenic acids and fromC. albicans with indigenous linolenic acid.
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Abbreviations
- AAPH:
-
2,2′-azobis(2-amidinopropane)dihydrochloride
- ACHN:
-
1,1′-azobis(4-cyclohexanecarbonitrile)
- CD:
-
conjugated dienes
- FAME:
-
fatty acid methyl esters
- PUFA:
-
oligounsaturated fatty acids
- TBHP:
-
tert-butyl hydroperoxide
- TBRS:
-
thiobarbituric acid-reactive substances
- YEPD:
-
yeast extract-peptone-dextrose
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Krasowska, A., Chmielewska, L., Prescha, A. et al. Viability and formation of conjugated dienes in plasma membrane lipids ofSaccharomyces cerevisiae, schizosaccharomyces pombe, rhodotorula glutinis andCandida albicans exposed to hydrophilic, amphiphilic and hydrophobic pro-oxidants. Folia Microbiol 47, 145–151 (2002). https://doi.org/10.1007/BF02817672
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DOI: https://doi.org/10.1007/BF02817672