Folia Microbiologica

, 45:331 | Cite as

Effect of cadmium and copper on the production of citric acid byAspergillus niger

  • K. Tsekova
  • D. Dentchev
  • D. Todorova


Copper and cadmium inhibited the growth as well as citric acid production (depending on the heavy metal concentrations) by citric-acid-producingAspergillus niger. Activity of citrate synthase was connected with citrate synthesis in the absence as well as in the presence of heavy metals. The activity of aconitase, and both NAD- and NADP-isocitrate dehydrogenases was strongly inhibited by copper. The contents of DNA and proteins in the cells decreased but the contents of lipids and polysaccharides increased considerably in the presence of both heavy metals.


Heavy Metal Cadmium Citric Acid Pyruvate Carboxylase Citric Acid Production 


  1. Anfinsen C.B.: Aconitase from pig heart muscle, pp. 695–698 in S.P. Colowick, N.O. Kaplan (Eds):Method in Enzymology, Vol. I. Academic Press, New York 1955.CrossRefGoogle Scholar
  2. Baldrian P., Gabriel J., Nerud F.: Effect of cadmium on the ligninolytic activity ofStereum hirsufum andPhanerochœte chrysosporium.Folia Microbiol. 41, 363–367 (1996).CrossRefGoogle Scholar
  3. Baldrian P., Gabriel J.: Effect of heavy metals on the growth of selected wood-rotting basidiomycetes.Folia Microbiol. 42, 521–523 (1997).Google Scholar
  4. Barton K.: A study of the conditions and mechanism of the diphenilamine reaction for the colorimetric estimation of deoxyribonucleic acid..Biochem. J. 62, 315–323 (1965).Google Scholar
  5. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities for the protein utilizing the principle of protein-dye binding.Analyt. Biochem. 72, 255–260 (1976).CrossRefGoogle Scholar
  6. Dagley S.: Citra. UV-spektrophotometrische Bestimmung, pp. 1520–1523 in H.U. Bergmyer (Ed.):Methoden der enzymatischen Analyse, Band III. Academie-Verlag, Berlin 1970.Google Scholar
  7. Hockertz S., Plonzig J., Auling G.: Impairment of DNA formation is an early event inAspergillus niger under manganese starvation.Appl. Microbiol. Biotechnol. 25, 590–593 (1987).CrossRefGoogle Scholar
  8. Kubicek C.P., Röhr M.: The influence of manganese on enzyme synthesis and citric acid accumulation inAspergillus niger.Eur J. Appl. Microbiol. Biotechnol. 4, 167–175 (1977).Google Scholar
  9. Kubicek C.P., Röhr M.: Novel trends in physiology and technology of citric acid production, pp. 253–262 in V. Krumphanzl (Ed.):Overproduction of Microbial. Products. Academic Press, London 1982.Google Scholar
  10. Kubicek C.P., Röhr M.: The citric acid fermentation.CRC Crit. Rev. Biotechnol. 3, 331–373 (1986).CrossRefGoogle Scholar
  11. Kuboye O.A., Andersen J.G., Smith J.E.: Control and autolysis of a spherical cell fromAspergillus niger.Trans. Brit. Mycol. Soc. 1, 27–31 (1976).CrossRefGoogle Scholar
  12. La Nause J.: Aconitase and isocitric dehydrogenase ofAspergillus niger in relation to citric acid production.J. Gen. Microbiol. 44, 73–81 (1966).Google Scholar
  13. Legisa M., Kidric J.: Initiation of citric acid accumulation in the early stages ofAspergillus niger growth.Appl. Microbiol. Biotechnol. 31, 453–457 (1989).CrossRefGoogle Scholar
  14. Prasad R., Rose A.H.: Involvement of lipids in solute transport in yeasts.Yeast 2, 205–220 (1986).PubMedCrossRefGoogle Scholar
  15. Röhr M., Kubicek C.P.: Regulatory aspects of citric acid fermentation byAspergillus niger.Proc. Biochem. 6, 34–37 (1981).Google Scholar
  16. Rozicki H.: Effect of heavy metals (Pb, Zn, Cu and Cd) on germination of conidia ofCylindrocarpon destructans (Zinssm). Scholten.Zbl. Mikrobiol. 147, 261–269 (1992).Google Scholar
  17. Sanglimsuwan S., Yoshida N., Morinaga T., Murooka Y.: Resistance to and uptake of heavy metals in mushrooms.J. Ferment. Bioeng. 75, 112–114 (1993).CrossRefGoogle Scholar
  18. Schmitz S., Weidenborner M., Kunz B.: Heavy metals as selected inhibitors of mould growth.Internat. J. Food Microbiol. 18, 233–236 (1993).CrossRefGoogle Scholar
  19. Srere P.A., Kosicki G.W.: The purification of citrate condensing enzyme.J. Biol. Chem. 236, 2557–2563 (1961).PubMedGoogle Scholar
  20. Trevelyan W.E., Harrison S.: Studies on yeast metabolism.Biochem. J. 50, 298–302 (1952).PubMedGoogle Scholar
  21. Wongchai V., Jeferson W.E.: Pyruvate carboxylase inAspergillus niger.Fed. Proc. Fed. Am. Soc. Exp. Biol. 33, 1378–1383 (1974).Google Scholar

Copyright information

© Folia Microbiologica 2000

Authors and Affiliations

  • K. Tsekova
    • 1
  • D. Dentchev
    • 1
  • D. Todorova
    • 1
  1. 1.Institute of MicrobiologyBulgarian Academy of SciencesSofiaBulgaria

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