Folia Microbiologica

, Volume 40, Issue 1, pp 118–122 | Cite as

Immobilization of pectinase fromSclerotium rolfsii

  • P. S. Channe
  • J. G. Shewale


Immobilization of pectinase fromS. rolfsii was studied on different matrices of which Amberlite XAD-7 showed maximum adsorption and expression of the enzyme. The most active preparation was obtained when XAD-7 was activated with 2% glutaraldehyde and 1.7 μkat of enzyme per g resin was used for immobilization at pH 5.5 and 28°C. Optimum pH and temperature of theS. rolfsii pectinase remain unaltered, 3.5 and 55°C, respectively, after immobilization. However, the apparentK M value of the enzyme decreased from 1.75 g/L for soluble enzyme preparation to 1.4 g/L for immobilized enzyme preparation. Both soluble and immobilized enzyme preparations were most stable at pH 4.0. The immobilized enzyme preparation was more stable than the soluble enzyme.


Pectin Immobilize Enzyme Soluble Enzyme Adsorbed Enzyme Pectin Solution 
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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1995

Authors and Affiliations

  • P. S. Channe
    • 1
  • J. G. Shewale
    • 1
  1. 1.Research and DevelopmentHindustan Antibiotics Ltd., PimpriPuneIndia

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