Folia Microbiologica

, Volume 40, Issue 1, pp 104–110 | Cite as

Effect of external pH on yield, morphology and cell components ofCandida utilis growing in continuous culture

  • J. Votruba
  • D. Vraná


The physiological state ofCandida utilis growing in continuous culture was controlled by external pH and dilution rate. The pH of the medium influences the physiological mechanisms coupled with the maintenance of living functions. The consumption of nitrogen source for biomass formation is a linear function of growth rate, independent of external pH. The statistical distribution of cell volumes was identified as log-normal where the dispersion is indirectly proportional to the maintenance coefficient and the mean of the logarithm of cell volume is a linear combination of specific growth rate and maintenance coefficient. The content of RNA, protein, glucan and mannan in dry biomass may also be expressed as a simple function of specific growth rate and maintenance coefficient.


Specific Growth Rate Glucan Dilution Rate Yield Coefficient Chemostat Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

List of symbols


dilution rate, 1/h


parameter, defined in Eq. 3


probability distribution, defined by Eq. 5


negative logarithm of dissociation constant


maintenance coefficient, 1/h


volume of cell, calculated by Eq. 1


yield coefficient

α, β

parameters of log-normal distribution (Eq. 5)


specific growth rate, 1/h


mass percent of cell component, based on dry biomass









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Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1995

Authors and Affiliations

  • J. Votruba
    • 1
  • D. Vraná
    • 1
  1. 1.Institute of MicrobiologyAcademy of Sciences of Czech RepublicPrague 4

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