Abstract
Novel corynebacterial plasmids carrying α-amylase gene fromBacillus have been constructed. The level of α-amylase expression depends on the size of the vector. The highest expression levels were measured in brevibacteria harboring pA61 plasmid.
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Barák I., Koptides M., Jucovič M., Šišová M., Timko J.: Construction of a promoter-probe shuttle vector forEscherichia coli and brevibacteria.Gene 95, 133–135 (1990).
Cadenas R.F., Gil J.A., Martin J.F.: Expression ofStreptomyces genes encoding extracellular enzymes inBrevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as inStreptomyces lividans.Appl. Microbiol. Biotechnol. 38, 362–369 (1992).
Folletie M.T., Sinskey A.J.: Recombinant DNA technology forCorynebacterium glutamicum.Food Technol. 40, 88–94 (1986).
Jucovič M., Ugorčáková J., Barák I., Timko J.: Transformation of amino acid-producing brevibacteria with recombinant plasmids carrying the alpha-amylase gene.Biológia, (Bratislaya)46, 281–288 (1991a).
Jucovič M., Pecháň P., Barák I., Ugorčáková J., Timko J.: Transfer of genes for synthesis of polysaccharide-cleaving enzymes into coryneform bacteria, pp. 153–160 in J. Balan (Ed.):Metabolism and Enzymology of Nucleic Acids Including Gene and Protein Engineering 7 (Symposium), Bratislava 1991b.
Martin J.F., Santamaria R., Sandoval H., Del Real G., Mateos L.M., Gil J.A., Aguilar A.C.: Cloning systems in amino acid-producing corynebacteria.Bio./Technology 5, 137–146 (1987).
Sambrook J., Fritsch E.F., Maniatis T.:Molecular cloning—a Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor (New York) 1989.
Santamaria R.I., Gil J.A., Martin J.F.: Characterization of an endogenous plasmid and development of cloning vectors and a transformation system inBrevibacterium lactofermentum.J. Gen. Microbiol. 130, 2237–2246 (1985).
Schäfer A., Kalinowski J., Simon R., Seep-Feldhaus A.H., Pūhler A.: High-frequency conjugal plasmid transfer from gram-negativeEscherichia coli to various gram-positive coryneform bacteria.J. Bacteriol. 172, 1663–1666 (1990).
Slugeňová M., Bukovská G., Timko J., Zeliková E., Zelinka J.: Molecular cloning of the α-amylase gene from an industrial production strain.Biológia (Bratislava)43, 1055–1067 (1988).
Smith M.D., Flickinger J.L., Lineberger D.W., Schmidt B.: Protoplast transformation in coryneform bacteria and introduction of α-amylase gene fromBacillus amyloliquefaciens intoBrevibacterium lactofermentum.Appl. Environ. Microbiol. 51, 634–639 (1986).
Somogyi M.: Notes on sugar determination.J. Biol. Chem. 195, 19–22 (1952).
Sorokin A.V., Iomantas Y.V., Strongin A.Y., Kozlov Y.I., Stepanov A.I., Debabov V.G.: Synthesis and secretion ofB. amyloliquefaciens active α-amylase inB. subtilis andE. coli cells.Mol. Genet. Microbiol. Virusol. 6, 24–27 (1984).
Twigg A.J., Sherratt D.: Trans-complementable copy-number mutants of plasmid ColE1.Nature 283, 216 (1980).
Yeh P., Oreglia J., Prévos F., Sicard A.M.: A shuttle vector system forBrevibacterium lactofermentum.Gene 47, 301–306 (1986).
Yoshihama M., Higashiro K., Rao E.A., Akedo M., Shanabruch W.G., Follettie M.T., Walker G.C., Sinskey A.J.: Cloning vector system forCorynebacterium glutamicum.J. Bacteriol. 162, 591–597 (1985).
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Ugorčáková, J., Jucovič, M., Bukovská, G. et al. Construction and characterization of new corynebacterial plasmids carrying the α-amylase gene. Folia Microbiol 41, 10–14 (1996). https://doi.org/10.1007/BF02816332
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DOI: https://doi.org/10.1007/BF02816332