Folia Microbiologica

, Volume 44, Issue 2, pp 196–200 | Cite as

Growth and siderophore production inBradyrhizobium (lupin) strains under iron limitation

  • M. H. Abd-Alla


SixBradyrhizobium (lupin) strains were evaluated for their ability to produce siderophores using four chemical assays. Two strains gave positive reactions with chrome azurol S assay (CAS) and produced hydroxamate-type siderophores. The other four strains gave negative results for siderophore production using the four assays. Generation time, growth yield and hydroxamate production of one strain (WPBS 3201 D) were affected by the iron concentration of the culture medium and the previous culture history of the cells. Resuspension of washed cells grown previously in media supplemented with 0 and 20 μmol/L Fe into differing iron regimes (0, 0.5, 1, 2, 4, 8, 10, 15 and 20 μmol/L Fe) suggest that the extent of hydroxamate production depended on the growth history of the cells. Cells pregrown in 20 μmol/L Fe produced a high amount of hydroxamates compared with cells pregrown in iron-free medium when resuspended in medium containing up to 4 μmol/L Fe. Cells pregrown in 20 μmol/L Fe were more sensitive to iron repression than those pregrown in 0.5 μmol/L Fe. Mannitol was the best carbon source for siderophore production. Siderophore synthesis was inhibited by 4-chloromercuribenzenesulfonic acid, 2,4-dinitrophenol, sodium azide and MgCl2 suggesting that an energized membrane and a mercapto group are essential and required for hydroxamate synthesis in strain WPB5 3201 D.


Iron Deficiency Siderophore Production Minimal Salt Medium Mercapto Group Siderophore Synthesis 
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  1. Alexander B.D., Zuberer D.A.: Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria.Biol. Fert. Soils12, 39–45 (1991).CrossRefGoogle Scholar
  2. Brown C.M., Dilworth M.J.: Ammonia assimilation byRhizobium cultures and bacteroids.J. Gen. Microbiol.86, 39–48 (1975).PubMedGoogle Scholar
  3. Carrillo-Castaneda G., Peralta J.R.V.: Siderophore-like activies inRhizobium phaseoli.J. Plant Nutr.11, 935–944 (1988).Google Scholar
  4. Carson K.C., Dilworth M.J., Glenn A.R.: Siderophore production and iron transport inRhizobium leguminosarum bv.viciae MNF710.J. Plant. Nutr.15, 2203–2220 (1992).Google Scholar
  5. Crowley D.E., Reid C.C.P., Szanislo P.J.: Microbial siderophores as iron sources for plants, pp. 375–386 in G. Winkelman, D. Van der Helm, J.B. Neilands (Eds):Iron Transport in Microbes, Plant and Animals. VCH Publishers, New York 1987.Google Scholar
  6. Eady R.R., Postgate J.R.: Nitrogenase.Nature249, 805–819 (1974).PubMedCrossRefGoogle Scholar
  7. Francis A.J., Alexander M.: Catalase activity and nitrogen fixation in legume root nodules.Can. J. Microbiol.18, 861–864 (1972).PubMedCrossRefGoogle Scholar
  8. Gibson F., Margrath D.I.: The isolation and characterization of hydroxamic acid (aerobactin) formed byAerobacter aerogenes 62-1.Biochim. Biophys. Acta192, 175–184 (1969).PubMedGoogle Scholar
  9. Guerinot M.L.: Iron uptake and metabolism in the rhizobia/legume symbioses.Plant & Soil130, 199–209 (1991).CrossRefGoogle Scholar
  10. Guerinot M.L., Meidl E.J., Plessner O.: Citrate as a siderophore inBradyrhizobium japonicum.J. Bacteriol.172, 3298–3303 (1990).PubMedGoogle Scholar
  11. Hemantraranjan A., Garg O.K.: Introduction of nitrogen fixing nodules throught iron and zinc fertilization in the non nodule-forming fernch bean (Phaseolus vulgaris L.).J. Plant Nutr.9, 281–288 (1986).Google Scholar
  12. Modi M., Shah K.S., Modi V.V.: Isolation and characterization of catechol-like siderophore from cowpeaRhizobium RA-1.Arch. Microbiol.141, 156–158 (1985).CrossRefGoogle Scholar
  13. Mollering H., Gruber W.: Determination of citrate with citrate lyase.Anal. Biochem.17, 369–376 (1966).CrossRefGoogle Scholar
  14. Neilands J.B.: Microbial envelope proteins related to iron.Ann. Rev. Microbiol.36, 285–309 (1982).CrossRefGoogle Scholar
  15. O'Hara G.W., Dilworth M.J., Bookerd N., Parkpian P.: Iron deficiency specifically limits nodule development in peanut inoculated withBradyrhizobium sp.New Phytol.108, 51–57 (1988).CrossRefGoogle Scholar
  16. Rai R., Singh S.N., Prasad V.: Effect of presumed amended pyrite on symbiotic N2-fixation, active iron content of nodules, grain yield and quality of chickpea (Cicer arietinumLinn.) genotypes in calcareous soil.J. Plant Nutr.5, 905–913 (1982).Google Scholar
  17. Reeves M., Pine L., Neilands J.B., Ballows A.: Absence of siderophore activity inLegionella sp. grown in iron deficient media.J. Bacteriol.154, 324–329 (1983).PubMedGoogle Scholar
  18. Rioux C.R., Jordan D.C., Rattary J.B.M.: Iron requirement ofRhizobium leguminosarum and secration of anthranilic acid during growth on iron-deficient medium.Arch. Biochem. Biophys.248, 175–182 (1986).PubMedCrossRefGoogle Scholar
  19. Smith M.J., Neilands J.B.: Rhizobactin, a siderophore fromRhizobium meliloti.J. Plant Nutr.7, 449–458 (1984).CrossRefGoogle Scholar
  20. Tang C., Robson A.D., Dilworth M.J.: The role of iron in nodulation and nitrogen fixation inLupinus angustifolius L.New Phytol.114, 173–182 (1990).CrossRefGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1999

Authors and Affiliations

  1. 1.Department of Botany, Faculty of ScienceAssiut UniversityAssiutEgypt

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