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Folia Microbiologica

, 44:59 | Cite as

Effect of the flavonoid quercetin on culture and isolation ofFrankia fromCasuarina root nodules

  • W. F. Sayed
  • C. T. Wheeler
Papers

Abstract

The growth of threeFrankia strains (HFPCcl3, ORS021001, ORS020607), isolated from nodules ofCasuarina, was unaffected by 5–15 μmol/L quercetin. Re-isolation ofFrankia strain HFPCc13 fromCasuarina root nodules was carried out with the addition of 15 μmol/L quercetin to three different media. The flavonoid significantly reduced fungal contaminants in the isolation plates and increased the number ofFrankia colonies per plate compared to controls without the flavonoid.

Keywords

Flavonoid Quercetin Arbuscular Mycorrhizai Juglone Frankia Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Aguilar M.M., Ashby A.M., Richards A.J.M., Loake G.J., Watson M.D., Shaw C.H.: Chemotaxis ofRhizobium leguminosarum biovarphaseoli towards flavonoid inducers of the symbiotic nodulation genesJ. Gen. Microbiol. 134, 2741–2746 (1988).Google Scholar
  2. Baker D., O'Keefe D.: A modified sucrose fractionation procedure for the isolation of frankiae from actinorhizal root nodules and soil samples.Plant & Soil 78, 23–28 (1984).CrossRefGoogle Scholar
  3. Balaji B., Poulin M.J., Vierheilig H., Piche Y.: Responses of an arbuscular mycorrhizal fungus,Gigaspora margarita, to exudates and volatiles from the Ri T-DNA-transformed roots of nonmycorrhizal and mycorrhizal mutants ofPisum sativum L. Sparkle.Exp. Mycol. 19, 275–283 (1995).CrossRefGoogle Scholar
  4. Becard G., Douds D.D., Pfeffer P.E.: Extensivein vitro hyphal growth of vesicular arbuscular mycorrhizal fungi in the presence of CO2 and flavonols.Appl. Environ. Microbiol. 58, 821–825 (1995).Google Scholar
  5. Benson D. R.: Isolation ofFrankia, strains from alder actinorhizal root nodules.Appl. Environ. Microbiol. 44, 461–465 (1982).PubMedGoogle Scholar
  6. Blom J., Roelfson W., Akkermans A.D.L.: Growth ofFrankia AvcI1 on media containing Tween 80 as C-source.FEMS Microbiol.Lett. 9, 131–135 (1980).CrossRefGoogle Scholar
  7. Cooper J.E., Rao R.J.: Localized changes in flavonoid biosynthesis in roots ofLotus pedunculatus after infection byRhizobium loti.Plant Physiol. 100, 444–450 (1992).PubMedGoogle Scholar
  8. Diem H.G., Dommergues Y.: The isolation ofFrankia from nodules ofCasuarina.Can. J. Bot. 61, 2822–2825 (1983).Google Scholar
  9. Diem H.G., Gauthier D., Dommergues Y.: An effective strain ofFrankia fromCasuarina sp.Can. J. Bot. 61, 2815–2821 (1983).Google Scholar
  10. Harborne J.B.: Phenolic compounds, pp. 33–80 inPhytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Chapman & Hall, London 1973.Google Scholar
  11. Lalonde M., Calvert H.E.: Production ofFrankia, hyphae and spores as an infective inoculant forAlnus species, pp. 95–110 inSymbiotic Nitrogen Fixation in the Management of Temperature Forests (J. Gordon, C.T. Wheeler, D.A. Perry, Eds). Forest Research Laboratory, Oregon State University, Corvallis 1979.Google Scholar
  12. Malcolm D.C., Hooker J.E., Wheeler C.T.:Frankia symbiosis as a source of nitrogen in forestry: a case study of symbiotic nitrogen fixation in a mixedAlnus—Picea plantation in Scotland.Proc. Roy. Soc. 85B, 263–282 (1985).Google Scholar
  13. Natarajan S., Murti V.V.S., Seshadri T.R.: Chemotaxonomical studies of someCasuarina species.Phytochemistry 10, 1083–1085 (1971).CrossRefGoogle Scholar
  14. Nittayajarn A., Baker D.D.: Methods for the quantification ofFrankia cell biomass.Plant & Soil 118, 199–204 (1989).CrossRefGoogle Scholar
  15. Perradin Y.M., Mottet J., Lalonde M.: Influence of phenolics onin vitro growth ofFrankia strains.Can. J. Bot. 61, 2807–2814 (1983).CrossRefGoogle Scholar
  16. Picman A.K., Schneider E.F., Picman J.: Effect of flavonoids on mycelial growth ofVerticillium albo-atrum.Biochem. Syst. Ecol. 23, 7–8 (1995).Google Scholar
  17. Quispel A., Tak T.: Studies on the growth of the endophyte ofAlnus glutinosa (L.)Vill. in nutrient solutions.New Phytol. 81, 587–600 (1978).CrossRefGoogle Scholar
  18. Quispel A., Suendsen A.B., Schripsema J., Baas W.J., Erkelens C., Lugtenburg J.: Identification of dipterocarpol as isolation factor for the induction of primary isolation ofFrankia form root nodules ofAlnus glutinosa (L.)Gaertner.Mol. Plant-Microbe Interact. 2, 107 (1989).Google Scholar
  19. Rosbrook P.A., Burggraaf A.J.P., Reddell P.: A comparison of two methods and different media for isolatingFrankia fromCasuarina root nodules.Plant & Soil 120, 187–193 (1989).CrossRefGoogle Scholar
  20. Rovira A.D.: Plant root exudates and their influence upon soil microorganisms, pp. 170–186 inEcology of Soil-Borne Plant Pathogens, an International Symposium on Factors, Determining the Behaviour of Plant Pathogens in Soil (K.F. Baker, W.C. Snyder, Eds). University of California Press, Berkeley, Los Angeles 1965.Google Scholar
  21. Saleh N.A.M., El-Lakany M.H.: A quantitative variation in the flavonoids and phenolics of someCasuarina species.Biochem. Syst. Ecol. 7, 13–15 (1979).CrossRefGoogle Scholar
  22. Sayed W.F.: The effectivity of theFrankia-Casuarina symbiosis in relation to the effects of some environmental factors prevalent in Egypt.PhD Thesis. Dept. Bot. Fac. Sci., South Valley Univ. Qena, (Egypt) 1995.Google Scholar
  23. Seeley H.W., Van Demark P.J.:Selected Excercises from “Microbes in Action”, a Laboratory Manual of Microbiology, 3rd ed. W.H. Freeman & Co., San Francisco 1981.Google Scholar
  24. Siqueira J.O., Nair M.G., Hammerschmidt R., Safir G.R.: Significance of phenolic compounds in plant-soil-microbial systems.CRC Plant Sci. 10, 63–121 (1991).CrossRefGoogle Scholar
  25. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals, 11th ed. Merck & Co., Rahway, N.J. (USA) 1989.Google Scholar
  26. Tiller S.A., Parry A.D., Edwards R.: Changes in the accumulation of flavonoid and isoflavonoid conjugates associated with plant age and nodulation in alfalfa (Medicago sativa).Physiol. Plant 91, 27–36 (1994).CrossRefGoogle Scholar
  27. Vogel C.S., Dawson J.O.:In vitro growth of fiveFrankia isolates in the presence of four phenolic acids and juglone.Soil Biol. Biochem. 18, 227–231 (1986).CrossRefGoogle Scholar
  28. Zaat S.A.J., Spaink H.P., Wijffelman C.A., van Brussel A.A.N., Okker R.J.H., Lugtenburg B.J.J.: Flavonoid compounds as molecular signals inRhizobium-legume symbiosis, pp. 189–205 inCell to Cell Signals in Plant, Animal and Microbial Symbiosis (Scanneriniet al.). NATO ASI Series Vol. H17. Springer-Verlag, Berlin-Heidelberg 1988.Google Scholar
  29. Zhang Z., Lopez M.F., Torrey J.G.: A comparison of cultural characteristics and infectivity ofFrankia isolate from root nodules ofCasuarina species.Plant & Soil 87, 1–16 (1984).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1999

Authors and Affiliations

  • W. F. Sayed
    • 1
  • C. T. Wheeler
    • 2
  1. 1.Department of BotanyFaculty of ScienceQenaEgypt
  2. 2.Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life SciencesUniversity of GlasgowGlasgowScotland, UK

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