Molecular Neurobiology

, Volume 9, Issue 1–3, pp 253–258 | Cite as

Altered GABAergic and glutamatergic transmission in audiogenic seizure-susceptible mice

  • M. L. Cordero
  • J. G. Ortiz
  • G. Santiago
  • A. Negrón
  • J. A. Moreira


The C57BL/10 SPS/sps mouse mutant are audiogenic seizure-susceptible. The enzymatic activities of glutamate decarboxylase (GAD), GABA aminotransferase (GABA-T), alanine aminotransferase (ALA-T), aspartate aminotransferase (ASP-T), and glutamate dehydrogenase (GDH) of whole brain supernatant are significantly reduced in these epileptic mice. GABA uptake is decreased in cortex, midbrain, and pons medulla. Previous studies showed the presence of two sodium-dependent GLU uptake systems in normal (SPS/SPS) mice. Glutamate Umax by System 1 is significantly decreased in these mice, whereas the Umax value for System 2 is significantly increased in the epileptic mice.

Index Entries

Glutamate GABA audiogenic seizures epilepsy uptake release 



gamma-aminobutyric acid


l-glutamic acid


normal mice


epileptic (audiogenic seizure-susceptible) mice




excitatory amino acids


glutamate decarboxylase


GABA aminotransferase


alanine aminotransferase


aspartate aminotransferase


glutamate dehydrogenase


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  1. 1.
    Dingledine R., McBain C. J., and McNamara O. (1991)Trends Pharm. Sci. Special Report, 49–53.Google Scholar
  2. 2.
    Meldrum B. S. and Murugaiah K. (1983)Eur. J. Pharmacol. 89, 149–152.PubMedGoogle Scholar
  3. 3.
    Laird H. E. and Jobe P. C. (1987) inNeurotransmitters, and Epilepsy (Jobe P. C. and Laird H. E., eds.), Humana, Clifton, NJ, pp. 57–94.CrossRefGoogle Scholar
  4. 4.
    Van Gelder N. M., Sherwin A. L., and Rasmussen T. (1972)Brain Res. 40, 385–393.PubMedCrossRefGoogle Scholar
  5. 5.
    Maxson S. C., Fine M. D., Ginsburg B. E., and Koniecki D. (1983)Epilepsia 24, 15–24.PubMedCrossRefGoogle Scholar
  6. 6.
    Flavin H. J., Wieraszko A., and Seyfried T. N. (1991)J. Neurochem. 56, 1007–1011.PubMedCrossRefGoogle Scholar
  7. 7.
    Ortiz J. G., Negrón A. E., Thomas A. P., Heimer H., Moreira J. A., Cordero M. L., Aranda J., and Bruno M. S. (1991)Exp. Neurol. 113, 338–343.PubMedCrossRefGoogle Scholar
  8. 8.
    Sze P. Y. (1977)Brain Res. 122, 59–69.PubMedCrossRefGoogle Scholar
  9. 9.
    Dinwoodie R. C. and Boecker E. A. (1979)Analyt. Biochem. 96, 24–33.PubMedCrossRefGoogle Scholar
  10. 10.
    Robinson M. B., Hunter-Ensor M., and Sinor J. (1991)Brain Res. 544, 196–202.PubMedCrossRefGoogle Scholar
  11. 11.
    Bradford M. M. (1976)Analyt. Bochem. 72, 248–254.CrossRefGoogle Scholar
  12. 12.
    Cordero M. L., Ortiz J. G., and Santiago G. (1994)Analyt. Biochem. 78, 44–48.Google Scholar
  13. 13.
    Ortiz J. G., Cordero M. L., and Santiago G. (1993) Workshop on Neurobiology of Epilepy, Tromso, Norway, June 26–29.Google Scholar
  14. 14.
    Faingold C. L., Naritoku D. K., Copley C. A., Randall M. E., Riaz A., Boersma-Anderson C. A., and Arneric S. P. (1992)Epilepsy Res. 13, 95–105.PubMedCrossRefGoogle Scholar
  15. 15.
    Maxson S. C. and Cowen J. S. (1976)Physiol. Behav. 16, 623–629.PubMedCrossRefGoogle Scholar
  16. 16.
    Chapman A. G. (1993) Workshop on Neurobiology of Epilepsy, Tromso, Norway, June 26–29.Google Scholar
  17. 17.
    Kanai Y. and Hediger M. (1992)Nature 360, 467–469.PubMedCrossRefGoogle Scholar
  18. 18.
    Pines G., Danbolt N., Bjoras M., Zhang Y., Bendahan A., Elde L., Koepsell H., Storm-Mathisen J., Seeberg E., and Kanner B. I. (1992)Nature 360, 464–466.PubMedCrossRefGoogle Scholar
  19. 19.
    Storck T., Schulte S., Homann K., and Stoffel W. (1992)Proc. Natl. Acad. Sci. USA 89, 10,955–10,959.CrossRefGoogle Scholar
  20. 20.
    Uhl G. R. and Hartig P. R. (1992)Trends Pharm. Sci. 13, 421–425.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc 1994

Authors and Affiliations

  • M. L. Cordero
    • 1
  • J. G. Ortiz
    • 1
  • G. Santiago
    • 1
  • A. Negrón
    • 1
  • J. A. Moreira
    • 1
  1. 1.Department of PharmacologyUniversity of Puerto Rico, School of MedicineSan Juan

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