Advertisement

Folia Microbiologica

, Volume 42, Issue 6, pp 595–600 | Cite as

Culture growth and IAA production by a microbial diazotropic symbiont of stem-nodules of the legumeAeschynomene aspera

  • A. C. Ghosh
  • P. S. Basu
Papers

Abstract

The stem nodules of the legumeAeschynomene aspera contain indoleacetic acid and a high amount of tryptophan. TheAzorhizobium caulinodans isolated from the stem nodules of the leguminous emergent hydrophyte produced a high amount of IAA (14.8 mg/L) inl-tryptophan-supplemented basal medium. The IAA yields paralleled the culture growth rate and increased up to 52 h. No separate growth and production phase was observed. The IAA production was increased 344% when the medium was supplemented withl-tryptophan, sucrose, FeSO4·7H2O, NaNO3, ascorbic acid and sodium dodecyl sulfate. The possible role of the IAA production in the legume-bacterium symbiosis is discussed.

Keywords

Tryptophan Root Nodule Indoleacetic Acid Stem Nodule Yeast Extract Sucrose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Beltra R., Diaz F., Fraile G.: The formation of growth substances byRhizobium species.Zentr. Bakteriol. Parasitenkd. Infektionskr. Hyg. Abt. 2. Naturwiss. Mikrobiol. Landwirtsch. Technol. Umweltsch.135, 617–622 (1980).Google Scholar
  2. Bahttacharyya R.N., Basu P.S.: Studies of the root nodules of leguminous plants. IV. Production of indole acetic acid by aBradyrhizobium sp. from the root nodules of a leguminous shrubCrotalaria retusa L.Acta Biotechnol.11, 439–447 (1991).CrossRefGoogle Scholar
  3. Bhattacharyya R.N., Basu P.S.: Bioproduction of indole acetic acid by aRhizobium sp. from root nodules of a leguminous climber,Psophocarpus tetragonolobus DC.Ind. J. Exp. Biol.30, 632–635 (1992).Google Scholar
  4. Bhowmick P.K., Basu P.S.: Indole acetic acid production byRhizobium sp. from a leguminous treeSesbania grandifloraPers.Egypt. J. Microbiol.22, 293–301 (1987).Google Scholar
  5. Chattopadhyay K.K., Basu P.S.: Bioproduction of indole acetic acid by theRhizobium sp. from root nodules of a leguminous treeDalbergia sissooRoxe.Acta. Microbiol. Polon.38, 293–305 (1989).Google Scholar
  6. Clark A.G.: Indole acetic acid proeduction byAgrobacterium andRhizobium sp.Microbios11A (46), 29–35 (1974).Google Scholar
  7. Conn H.J., Jennison M.W., Weeks O.B.: Routine tests for the identification of bacteria, pp. 140–168 in H.J. Conn (Ed.):Manual of Microbiological Methods. McGraw-Hill Book Co., New York 1957.Google Scholar
  8. De Bruijn F.J.: The unusual symbiosis between the diazotrophic stem-nodulating bacteriumAzorhizobium caulinodans ORS 571 and its host, the tropical legumeSesbania rostrata, pp. 457–504 in T. Kosuge, E.W. Nester (Eds):Plant-Microbe Interaction (Molecular and Genetic Perspectives), Vol. 3. McGraw-Hill Publ. Co., New York 1989.Google Scholar
  9. Dreyfus B., Garcia J.L., Gillis M.: Characterization ofAzorhizobium caulinodans gen.nov. sp.nov., a stem-nodulating nitrogen-fixing bacterium isolated fromSesbania rostrata.Int. J. Syst. Bacteriol.38, 89–98 (1988).CrossRefGoogle Scholar
  10. Dullaart J.: Quantitative estimation of indoleacetic acid and indolecarboxylic acid in root nodules and roots ofLupinus luteus L.Acta Bot. Neerl.16, 222–230 (1967).Google Scholar
  11. Gordon S.A., Weber R.P.: Colorimetric estimation of indoleacetic acid.Plant Physiol.26, 192–195 (1951).PubMedGoogle Scholar
  12. Garcia-Rodriguez T., Gutierrez-Navarroa M., Jimenez R., Perez-Silva J.: Effects of legume root exudates on indoleacetic acid production byRhizobium meliloti.Polish J. Soil Sci.14, 45–52 (1981).Google Scholar
  13. Hamilton R.H., Bandurski R.S., Grisby B.H.: Isolation of indole-3-acetic acid from corn kernels and etiolated corn seedlings.Plant Physiol.36, 354–359 (1961).PubMedGoogle Scholar
  14. Hassan S.S.M.: Spectrophotometric method for simultaneous determination of tryptophan and tyrosine.Anal. Chem.47, 1429–1432 (1975).PubMedCrossRefGoogle Scholar
  15. Jordan D.C.: Rhizobiaceae, pp. 234–244 in N.R. Krieg, J.G. Holt (Eds):Bergey's Manual of Systematic Bacteriology, Vol. 1. Williams & Wilkins, Baltimore 1984.Google Scholar
  16. Markova A., Chanova D.: Effects of molybdenum and cobalt on nitrogen fixing activity ofRhizobium japonicum and soybean yield.Task. Veg. Sci.13, 187–190 (1984).Google Scholar
  17. Moore T.C.:Biochemistry and Physiology of Plant Hormones, 2nd ed. Springer-Verlag, New York 1989.Google Scholar
  18. Nitsch J.P.: Free auxin and free tryptophan in strawberry.Plant Physiol.30, 33–39 (1955).PubMedCrossRefGoogle Scholar
  19. Panse V.G., Sukhatme P.V.:Statistical Methods for Agricultural Workers, 4th ed., pp. 145–156. Indian Council of Agricultural Research, New Delhi 1985.Google Scholar
  20. Sinha B.K., Basu P.S.: Indole-3-acetic acid and its metabolism in root nodules ofPongamia pinnata (L.)Pierre.Biochem. Physiol. Pflanzen176, 218–227 (1981).Google Scholar
  21. Skerman V.B.D.:A Guide to Identification of the Genera of Bacteria, pp. 189–191. Williams & Wilkins, Baltimore 1959.Google Scholar
  22. Stoessl A., Venis M.A.: Determination of submicrogram levels of indole-3-acetic acid: A new, highly specific method.Anal. Biochem.34, 344–351 (1970).PubMedCrossRefGoogle Scholar
  23. Vincent J.M.: Root nodule symbiosis withRhizobium, pp. 265–341 in A. Quespel (Ed.):The Biology of Nitrogen Fixation. North-Holland Publ. Co., Amsterdam 1974.Google Scholar
  24. Wheeler C.T., Henson I.E., McLaughlin M.E.: Hormones in plant bearing actinomycete root nodules.Bot. Gaz. (Suppl.)140, S52-S57 (1979).CrossRefGoogle Scholar
  25. Yoshida S., Yatazawa M.: Species characteristics and cultural conditions as affecting to rhizobial production of IAA.Nippon Dojo Hiryogaku Zasshi44, 63–66 (1973).Google Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 1997

Authors and Affiliations

  • A. C. Ghosh
    • 1
  • P. S. Basu
    • 1
  1. 1.Department of BotanyBurdwan UniversityBurdwanIndia

Personalised recommendations