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Molecular and Chemical Neuropathology

, Volume 30, Issue 1–2, pp 95–100 | Cite as

Limited junctional diversity of Vδ5-Jδ1 rearrangement in multiple sclerosis patients

  • Jerzy S. Nowak
  • Grazyna Michałowska-Wender
  • Danuta Januszkiewicz
  • Mieczysław Wender
Original Articles

Abstract

T-cell receptor (TCR) δ gene repertoire, as assessed by Vδ-Jδ rearrangements, has been analyzed in nine multiple sclerosis (MS) cases and in 30 healthy individuals by seminested PCR technique. Among the Vδ-Jδ junctional diversities studied, the most striking result has been observed in Vδ5-Jδ1 rearrangement. The detection of repeated Vδ5-Jδ1 nucleotide sequences in all analyzed clones from seven out of nine patients studied proved the monoclonal nature of γ δ T-cells with Vδ5-Jδ1 rearrangement. The clonal nature of this rearrangement proved by PAGE and sequencing analysis may suggest an antigen-driven expansion of γδT cells and argues for a significant role of γδ T-cells with Vδ5-Jδ1 rearrangement in MS pathogenesis. However, it cannot be excluded that clonal expansion of these lymphocytes may represent secondary change to central nervous system damage.

Index Entries

Multiple sclerosis T-cell receptor γ δ gene rearrangement autoimmune disease lymphocytes 

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Copyright information

© Humana Press Inc. 1997

Authors and Affiliations

  • Jerzy S. Nowak
    • 1
  • Grazyna Michałowska-Wender
    • 2
  • Danuta Januszkiewicz
    • 1
  • Mieczysław Wender
    • 2
  1. 1.Institute of Human GeneticsPolish Academy of SciencesPoznańPoland
  2. 2.Department of NeurologyAcademy of Medical SciencesPoznańPoland

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